Identification of glycerophosphorylcholine in mussel ovarian extracts by two-dimensional nuclear magnetic resonance

Teresa W.M. Fan; Andrew N. Lane

doi:10.1016/0003-2697(92)90362-B

Identification of glycerophosphorylcholine in mussel ovarian extracts by two-dimensional nuclear magnetic resonance

Teresa W.M. Fan, Andrew N. Lane

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The abundance of the "phosphodiester" peak in differentiating or proliferating tissues, including reproductive organs and tumors, warrants further investigations of its metabolic role(s), which would require a rigorous confirmation of its identity. The assignment of this peak to glycerophosphorylcholine in ³¹P NMR spectra of biological samples has been largely based on chemical shift, which can result in ambiguities. We employed a combination of two-dimensional ³¹P¹H heteronuclear shift correlation and ¹H total correlation spectroscopies to trace the spin connectivities of glycerophosphorylcholine and thus to identify its structure directly from crude ovarian extracts of mussels without ambiguities and the need for extensive purification. This approach can be applied generally to the identification of molecules containing heteroatoms in crude tissue extracts.

Original language	English
Pages (from-to)	251-255
Number of pages	5
Journal	Analytical Biochemistry
Volume	206
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(92)90362-B
State	Published - Nov 1 1992

Bibliographical note

Funding Information:
We thank Dr. Richard Higashi for discussions and Drs. Gary Cherr and Muralidharan Pillai for their assistance on mussel histology. This research was supported in part by the Minerals Management Service of the U.S. Department of Interior (Grant 14-35-0001-30-471).

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(92)90362-B

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abstract = "The abundance of the {"}phosphodiester{"} peak in differentiating or proliferating tissues, including reproductive organs and tumors, warrants further investigations of its metabolic role(s), which would require a rigorous confirmation of its identity. The assignment of this peak to glycerophosphorylcholine in 31P NMR spectra of biological samples has been largely based on chemical shift, which can result in ambiguities. We employed a combination of two-dimensional 31P1H heteronuclear shift correlation and 1H total correlation spectroscopies to trace the spin connectivities of glycerophosphorylcholine and thus to identify its structure directly from crude ovarian extracts of mussels without ambiguities and the need for extensive purification. This approach can be applied generally to the identification of molecules containing heteroatoms in crude tissue extracts.",

author = "Fan, {Teresa W.M.} and Lane, {Andrew N.}",

note = "Funding Information: We thank Dr. Richard Higashi for discussions and Drs. Gary Cherr and Muralidharan Pillai for their assistance on mussel histology. This research was supported in part by the Minerals Management Service of the U.S. Department of Interior (Grant 14-35-0001-30-471).",

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AU - Lane, Andrew N.

N1 - Funding Information: We thank Dr. Richard Higashi for discussions and Drs. Gary Cherr and Muralidharan Pillai for their assistance on mussel histology. This research was supported in part by the Minerals Management Service of the U.S. Department of Interior (Grant 14-35-0001-30-471).

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N2 - The abundance of the "phosphodiester" peak in differentiating or proliferating tissues, including reproductive organs and tumors, warrants further investigations of its metabolic role(s), which would require a rigorous confirmation of its identity. The assignment of this peak to glycerophosphorylcholine in 31P NMR spectra of biological samples has been largely based on chemical shift, which can result in ambiguities. We employed a combination of two-dimensional 31P1H heteronuclear shift correlation and 1H total correlation spectroscopies to trace the spin connectivities of glycerophosphorylcholine and thus to identify its structure directly from crude ovarian extracts of mussels without ambiguities and the need for extensive purification. This approach can be applied generally to the identification of molecules containing heteroatoms in crude tissue extracts.

AB - The abundance of the "phosphodiester" peak in differentiating or proliferating tissues, including reproductive organs and tumors, warrants further investigations of its metabolic role(s), which would require a rigorous confirmation of its identity. The assignment of this peak to glycerophosphorylcholine in 31P NMR spectra of biological samples has been largely based on chemical shift, which can result in ambiguities. We employed a combination of two-dimensional 31P1H heteronuclear shift correlation and 1H total correlation spectroscopies to trace the spin connectivities of glycerophosphorylcholine and thus to identify its structure directly from crude ovarian extracts of mussels without ambiguities and the need for extensive purification. This approach can be applied generally to the identification of molecules containing heteroatoms in crude tissue extracts.

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Identification of glycerophosphorylcholine in mussel ovarian extracts by two-dimensional nuclear magnetic resonance

Abstract

Bibliographical note

ASJC Scopus subject areas

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