Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Rukhsana Sultana; H. Fai Poon; Jian Cai; William M. Pierce; Michael Merchant; Jon B. Klein; William R. Markesbery; D. Allan Butterfield

doi:10.1016/j.nbd.2005.10.004

Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Rukhsana Sultana, H. Fai Poon, Jian Cai, William M. Pierce, Michael Merchant, Jon B. Klein, William R. Markesbery, D. Allan Butterfield

Research output: Contribution to journal › Article › peer-review

342 Scopus citations

Abstract

Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3- phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid β-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.

Original language	English
Pages (from-to)	76-87
Number of pages	12
Journal	Neurobiology of Disease
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/j.nbd.2005.10.004
State	Published - Apr 2006

Bibliographical note

Funding Information:
The authors thank the University of Kentucky ADRC Clinical and Neuropathology Cores for providing the brain specimens used for this study. This work was supported in part by grants from NIH to D.A.B. [AG-05119; AG-10836], to W.R.M. [5P01-AG-05119], and to J.B.K. [HL-66358].

Keywords

ATP synthase alpha chain
Alpha enolase
Alzheimer's disease
Carbonic anhydrase II
Glyceraldehyde-3-phosphate dehydrogenase
Hippocampus
Redox proteomics
Voltage-dependent anion-selective channel protein 1

ASJC Scopus subject areas

Neurology

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title = "Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach",

abstract = "Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3- phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid β-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.",

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note = "Funding Information: The authors thank the University of Kentucky ADRC Clinical and Neuropathology Cores for providing the brain specimens used for this study. This work was supported in part by grants from NIH to D.A.B. [AG-05119; AG-10836], to W.R.M. [5P01-AG-05119], and to J.B.K. [HL-66358].",

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doi = "10.1016/j.nbd.2005.10.004",

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AU - Sultana, Rukhsana

AU - Poon, H. Fai

AU - Cai, Jian

AU - Pierce, William M.

AU - Merchant, Michael

AU - Klein, Jon B.

AU - Markesbery, William R.

AU - Butterfield, D. Allan

N1 - Funding Information: The authors thank the University of Kentucky ADRC Clinical and Neuropathology Cores for providing the brain specimens used for this study. This work was supported in part by grants from NIH to D.A.B. [AG-05119; AG-10836], to W.R.M. [5P01-AG-05119], and to J.B.K. [HL-66358].

PY - 2006/4

Y1 - 2006/4

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KW - Alzheimer's disease

KW - Carbonic anhydrase II

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Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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