Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Rukhsana Sultana, H. Fai Poon, Jian Cai, William M. Pierce, Michael Merchant, Jon B. Klein, William R. Markesbery, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

332 Scopus citations

Abstract

Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3- phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid β-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.

Original languageEnglish
Pages (from-to)76-87
Number of pages12
JournalNeurobiology of Disease
Volume22
Issue number1
DOIs
StatePublished - Apr 2006

Bibliographical note

Funding Information:
The authors thank the University of Kentucky ADRC Clinical and Neuropathology Cores for providing the brain specimens used for this study. This work was supported in part by grants from NIH to D.A.B. [AG-05119; AG-10836], to W.R.M. [5P01-AG-05119], and to J.B.K. [HL-66358].

Keywords

  • ATP synthase alpha chain
  • Alpha enolase
  • Alzheimer's disease
  • Carbonic anhydrase II
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Hippocampus
  • Redox proteomics
  • Voltage-dependent anion-selective channel protein 1

ASJC Scopus subject areas

  • Neurology

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