Identification of Streptomyces olivaceus Tü 2353 genes involved in the production of the polyketide elloramycin

H. Decker; J. Rohr; H. Motamedi; H. Zähner; C. R. Hutchinson

doi:10.1016/0378-1119(95)00573-7

Identification of Streptomyces olivaceus Tü 2353 genes involved in the production of the polyketide elloramycin

H. Decker, J. Rohr, H. Motamedi, H. Zähner, C. R. Hutchinson

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The genes for the production of elloramycin (ELM) from Streptomyces olivaceus (So) Tü2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So Tü2353 conferred tetracenomycin C and ELM resistance to S. lividans TK64 and complemented a mutation in So Tü2353R. Introduction of cosmid 16F4 into S. lividans TK64 resulted in the production of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesis.

Original language	English
Pages (from-to)	121-126
Number of pages	6
Journal	Gene
Volume	166
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00573-7
State	Published - Dec 1 1995

Bibliographical note

Funding Information:
We are grateful to D.A. Hopwood for providing S. lividans TK64 and A. Bechthold for the plasmid containing the graE gene from S. violaceoruber Tii22. Thiostrepton was a gift from S.J. Lucania (Bristol-Myers Squibb), pKC505 from R. Nagaraja Rao (Eli Lilly) and TCM C as well as ELM A from H.P. Fiedler (Universit~it Tfibingen). We like to thank Sigrid Stockert and Nicole Fecher for excellent technical assistance. This work was possible by the financial support of the DFG (SFB 323) and the National Institute of Health (CA 35381) for work carried out at the University of Wisconsin.

Keywords

Recombinant DNA
cosmid
glycosylation
hybrid antibiotic
tetracenomycin
urdamycin

ASJC Scopus subject areas

Genetics

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title = "Identification of Streptomyces olivaceus T{\"u} 2353 genes involved in the production of the polyketide elloramycin",

abstract = "The genes for the production of elloramycin (ELM) from Streptomyces olivaceus (So) T{\"u}2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So T{\"u}2353 conferred tetracenomycin C and ELM resistance to S. lividans TK64 and complemented a mutation in So T{\"u}2353R. Introduction of cosmid 16F4 into S. lividans TK64 resulted in the production of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesis.",

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author = "H. Decker and J. Rohr and H. Motamedi and H. Z{\"a}hner and Hutchinson, {C. R.}",

note = "Funding Information: We are grateful to D.A. Hopwood for providing S. lividans TK64 and A. Bechthold for the plasmid containing the graE gene from S. violaceoruber Tii22. Thiostrepton was a gift from S.J. Lucania (Bristol-Myers Squibb), pKC505 from R. Nagaraja Rao (Eli Lilly) and TCM C as well as ELM A from H.P. Fiedler (Universit~it Tfibingen). We like to thank Sigrid Stockert and Nicole Fecher for excellent technical assistance. This work was possible by the financial support of the DFG (SFB 323) and the National Institute of Health (CA 35381) for work carried out at the University of Wisconsin.",

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AU - Rohr, J.

AU - Motamedi, H.

AU - Zähner, H.

AU - Hutchinson, C. R.

N1 - Funding Information: We are grateful to D.A. Hopwood for providing S. lividans TK64 and A. Bechthold for the plasmid containing the graE gene from S. violaceoruber Tii22. Thiostrepton was a gift from S.J. Lucania (Bristol-Myers Squibb), pKC505 from R. Nagaraja Rao (Eli Lilly) and TCM C as well as ELM A from H.P. Fiedler (Universit~it Tfibingen). We like to thank Sigrid Stockert and Nicole Fecher for excellent technical assistance. This work was possible by the financial support of the DFG (SFB 323) and the National Institute of Health (CA 35381) for work carried out at the University of Wisconsin.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - The genes for the production of elloramycin (ELM) from Streptomyces olivaceus (So) Tü2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So Tü2353 conferred tetracenomycin C and ELM resistance to S. lividans TK64 and complemented a mutation in So Tü2353R. Introduction of cosmid 16F4 into S. lividans TK64 resulted in the production of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesis.

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KW - Recombinant DNA

KW - cosmid

KW - glycosylation

KW - hybrid antibiotic

KW - tetracenomycin

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Identification of Streptomyces olivaceus Tü 2353 genes involved in the production of the polyketide elloramycin

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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