Identification of Streptomyces olivaceus Tü 2353 genes involved in the production of the polyketide elloramycin

H. Decker, J. Rohr, H. Motamedi, H. Zähner, C. R. Hutchinson

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


The genes for the production of elloramycin (ELM) from Streptomyces olivaceus (So) Tü2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So Tü2353 conferred tetracenomycin C and ELM resistance to S. lividans TK64 and complemented a mutation in So Tü2353R. Introduction of cosmid 16F4 into S. lividans TK64 resulted in the production of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesis.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
Issue number1
StatePublished - Dec 1 1995

Bibliographical note

Funding Information:
We are grateful to D.A. Hopwood for providing S. lividans TK64 and A. Bechthold for the plasmid containing the graE gene from S. violaceoruber Tii22. Thiostrepton was a gift from S.J. Lucania (Bristol-Myers Squibb), pKC505 from R. Nagaraja Rao (Eli Lilly) and TCM C as well as ELM A from H.P. Fiedler (Universit~it Tfibingen). We like to thank Sigrid Stockert and Nicole Fecher for excellent technical assistance. This work was possible by the financial support of the DFG (SFB 323) and the National Institute of Health (CA 35381) for work carried out at the University of Wisconsin.


  • Recombinant DNA
  • cosmid
  • glycosylation
  • hybrid antibiotic
  • tetracenomycin
  • urdamycin

ASJC Scopus subject areas

  • Genetics


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