Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

June Sun Yoon; Dipak K. Sahoo; Indu B. Maiti; Subba R. Palli

doi:10.1038/s41598-018-32742-2

Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

June Sun Yoon, Dipak K. Sahoo, Indu B. Maiti, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids [Methoprene-tolerant (Met), retinoid X receptor β, farnesoic acid O-methyltransferase, and CREB-binding protein] caused 35–56% mortality. Transgenic tobacco plants expressing hairpin dsRNA targeting Met gene were generated and tested. About 48% mortality was observed in TSSM raised on transgenic tobacco plants expressing dsMet. These studies not only broaden our knowledge on understanding hormone action in TSSM but also identified target genes that could be used in RNAi-mediated control of TSSM.

Original language	English
Article number	14687
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-32742-2
State	Published - Dec 1 2018

Bibliographical note

Publisher Copyright:
© 2018, The Author(s).

Funding

Research in the S.R.P. laboratory is supported by a grants from the National Institutes of Health (GM070559-12 and 1R21AI131427-01), the National Science Foundation (Industry/University Cooperative Research Centers, the Center for Arthropod Management Technologies under Grant IIP-1338776), and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2351177000). This is publication number 18-08-086 from the Kentucky Agricultural Experimental Station and published with the approval of the director. The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

Funders	Funder number
Center for Arthropod Management Technologies	IIP-1338776
Industry/University Cooperative Research Centers
US Department of Agriculture
National Science Foundation Arctic Social Science Program	IIP-1338775
National Institutes of Health (NIH)	1R21AI131427-01
National Institute of General Medical Sciences	R01GM070559
U.S. Department of Agriculture	2351177000
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

ASJC Scopus subject areas

General

Access to Document

10.1038/s41598-018-32742-2

Cite this

@article{8000596951ee4ed6b91f97e359bbbfc1,

title = "Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite",

abstract = "RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids [Methoprene-tolerant (Met), retinoid X receptor β, farnesoic acid O-methyltransferase, and CREB-binding protein] caused 35–56% mortality. Transgenic tobacco plants expressing hairpin dsRNA targeting Met gene were generated and tested. About 48% mortality was observed in TSSM raised on transgenic tobacco plants expressing dsMet. These studies not only broaden our knowledge on understanding hormone action in TSSM but also identified target genes that could be used in RNAi-mediated control of TSSM.",

author = "Yoon, {June Sun} and Sahoo, {Dipak K.} and Maiti, {Indu B.} and Palli, {Subba R.}",

note = "Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41598-018-32742-2",

language = "English",

volume = "8",

number = "1",

}

TY - JOUR

T1 - Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

AU - Yoon, June Sun

AU - Sahoo, Dipak K.

AU - Maiti, Indu B.

AU - Palli, Subba R.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids [Methoprene-tolerant (Met), retinoid X receptor β, farnesoic acid O-methyltransferase, and CREB-binding protein] caused 35–56% mortality. Transgenic tobacco plants expressing hairpin dsRNA targeting Met gene were generated and tested. About 48% mortality was observed in TSSM raised on transgenic tobacco plants expressing dsMet. These studies not only broaden our knowledge on understanding hormone action in TSSM but also identified target genes that could be used in RNAi-mediated control of TSSM.

AB - RNA interference (RNAi) is being developed for the management of pests that destroy crops. The twospotted Spider Mite (TSSM), Tetranychus urticae is a worldwide pest due to its unique physiological and behavioral characteristics including extraordinary ability to detoxify a wide range of pesticides and feed on many host plants. In this study, we conducted experiments to identify target genes that could be used for the development of RNAi-based methods to control TSSM. Leaf disc feeding assays revealed that knockdown in the expression genes coding for proteins involved in the biosynthesis and action of juvenile hormone (JH) and action of ecdysteroids [Methoprene-tolerant (Met), retinoid X receptor β, farnesoic acid O-methyltransferase, and CREB-binding protein] caused 35–56% mortality. Transgenic tobacco plants expressing hairpin dsRNA targeting Met gene were generated and tested. About 48% mortality was observed in TSSM raised on transgenic tobacco plants expressing dsMet. These studies not only broaden our knowledge on understanding hormone action in TSSM but also identified target genes that could be used in RNAi-mediated control of TSSM.

UR - http://www.scopus.com/inward/record.url?scp=85054196811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054196811&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-32742-2

DO - 10.1038/s41598-018-32742-2

M3 - Article

C2 - 30279530

AN - SCOPUS:85054196811

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 14687

ER -

Identification of target genes for RNAi-mediated control of the Twospotted Spider Mite

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this