TY - JOUR
T1 - Identifying loci required for follicular patterning using directed mosaics
AU - Duffy, Joseph B.
AU - Harrison, Douglas A.
AU - Perrimon, Norbert
PY - 1998
Y1 - 1998
N2 - We have developed a 'directed mosaic' system in Drosophila by using the GAL4 system to control the expression of the yeast recombinase, FLP, in a spatial and temporal fashion. By directing FP expression, we show that it is possible to efficiently and specifically target loss-of-function studies for vital loci to the developmental pathway of interest. A simple F1 adult phenotypic screen demonstrated that most adult tissues can be analysed with this approach. Using GAL4 lines expressed during oogenesis, we have refined the system to examine the roles of vital loci in the development of the follicular epithelium. We have identified essential genes involved in egg chamber organization, cell migration and cell shape. Further, we have used this technique to gain insights into the role of the Drosophila EGF receptor pathway in establishing the egg axes. Finally, using different UAS-FLP, GAL4 and existing FRT lines, we have built stocks that permit the analysis of ~ 95% of the genome in follicular mosaics.
AB - We have developed a 'directed mosaic' system in Drosophila by using the GAL4 system to control the expression of the yeast recombinase, FLP, in a spatial and temporal fashion. By directing FP expression, we show that it is possible to efficiently and specifically target loss-of-function studies for vital loci to the developmental pathway of interest. A simple F1 adult phenotypic screen demonstrated that most adult tissues can be analysed with this approach. Using GAL4 lines expressed during oogenesis, we have refined the system to examine the roles of vital loci in the development of the follicular epithelium. We have identified essential genes involved in egg chamber organization, cell migration and cell shape. Further, we have used this technique to gain insights into the role of the Drosophila EGF receptor pathway in establishing the egg axes. Finally, using different UAS-FLP, GAL4 and existing FRT lines, we have built stocks that permit the analysis of ~ 95% of the genome in follicular mosaics.
KW - Drosophila
KW - Follicle cells
KW - Mosaics
KW - Oogenesis
KW - UAS-FLP
UR - http://www.scopus.com/inward/record.url?scp=0031816410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031816410&partnerID=8YFLogxK
U2 - 10.1242/dev.125.12.2263
DO - 10.1242/dev.125.12.2263
M3 - Article
C2 - 9584125
AN - SCOPUS:0031816410
SN - 0950-1991
VL - 125
SP - 2263
EP - 2271
JO - Development (Cambridge)
JF - Development (Cambridge)
IS - 12
ER -