IFN-γ production is a hallmark of Th1 response, and IFN-γ has multiple roles in Th1 development, depending on the experimental conditions. In this study, the correlation between IFN-γ and interleukin 12 receptor β2 (IL-12Rβ2) expression was investigated in experimental autoimmune uveitis (EAU) susceptible B10.A and resistant BALB/c mice. B10.A mice expressed high IL-12Rβ2 on T cells either in the disease target eyes or draining lymph node cells (LNC), and its expression correlated with the Th1-type response. In contrast, BALB/c mice only expressed minimal IL-12Rβ2 in draining LNC and had lower Th1-response. B10.A mice produced more IFN-γ and generated a higher number of Th1 cells than that of BALB/c in the draining LNC. Furthermore, IL-12Rβ2 expression and STAT4 signaling were inhibited by anti-IFN-γ mAb in the cultured draining LNC from B10.A mice, but enhanced by adding exogenous IFN-γ in the cultured cells from BALB/c mice. The IL-12Rβ2 expression on Th1 cells from draining LNC was increased in the presence of IL-12 and IFN-γ. In conclusion, IFN-γ production correlated with IL-12Rβ2 expression on Th1-cells and IFN-γ had a potential to regulate IL-12Rβ2 expression in vivo. This regulatory mechanism might be involved in EAU induction.
|Number of pages||10|
|Journal||Journal of Neuroimmunology|
|State||Published - Apr 2003|
Bibliographical noteFunding Information:
We are grateful to Dr. Chen Dong (University of Washington, USA) for reviewing the manuscript and for helpful comments. This work was supported by 973 project (G1999053907), National Natural Science Foundation of China (30070708) and key Projects of Chinese Academy of Sciences (KSCX2-2-08-02) and National Key Basic Research Program of China (2001CB510006).
- IL-12 receptor beta 2
ASJC Scopus subject areas
- Immunology and Allergy
- Clinical Neurology