IL-4 induces apoptosis of endothelial cells through the caspase-3-dependent pathway

Yong Woo Lee; Hartmut Kühn; Bernhard Hennig; Michal Toborek

doi:10.1016/S0014-5793(00)02208-0

IL-4 induces apoptosis of endothelial cells through the caspase-3-dependent pathway

Yong Woo Lee, Hartmut Kühn, Bernhard Hennig, Michal Toborek

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The present study was designed to investigate the hypothesis that interleukin-4 (IL-4) can induce apoptosis of human endothelial cells and to study regulatory pathways of this process. Indeed, DNA ladder assay and flow cytometry study showed that IL-4 can induce apoptosis of endothelial cells in a time- and dose-dependent manner. In addition, IL-4 markedly increased activity of caspase-3, and inhibition of this enzyme suppressed IL-4-induced apoptosis in a dose-dependent manner. These results provide the first evidence that IL-4 can induce apoptosis of human endothelial cells. In addition, the data indicate that the caspase-3-dependent pathway is critically involved in this process.

Original language	English
Pages (from-to)	122-126
Number of pages	5
Journal	FEBS Letters
Volume	485
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(00)02208-0
State	Published - Nov 24 2000

Bibliographical note

Funding Information:
This work was supported in part by the Alexander von Humboldt Foundation and by research grants from the Deutsche Forschungsgemeinschaft, American Heart Association, and National Institutes of Health.

Publisher Copyright:
© 2000 Federation of European Biochemical Societies.

Keywords

Apoptosis
Atherosclerosis
Caspase-3
Human endothelial cell
Interleukin-4

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)02208-0

Cite this

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title = "IL-4 induces apoptosis of endothelial cells through the caspase-3-dependent pathway",

abstract = "The present study was designed to investigate the hypothesis that interleukin-4 (IL-4) can induce apoptosis of human endothelial cells and to study regulatory pathways of this process. Indeed, DNA ladder assay and flow cytometry study showed that IL-4 can induce apoptosis of endothelial cells in a time- and dose-dependent manner. In addition, IL-4 markedly increased activity of caspase-3, and inhibition of this enzyme suppressed IL-4-induced apoptosis in a dose-dependent manner. These results provide the first evidence that IL-4 can induce apoptosis of human endothelial cells. In addition, the data indicate that the caspase-3-dependent pathway is critically involved in this process.",

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author = "Lee, {Yong Woo} and Hartmut K{\"u}hn and Bernhard Hennig and Michal Toborek",

note = "Funding Information: This work was supported in part by the Alexander von Humboldt Foundation and by research grants from the Deutsche Forschungsgemeinschaft, American Heart Association, and National Institutes of Health. Publisher Copyright: {\textcopyright} 2000 Federation of European Biochemical Societies.",

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AU - Kühn, Hartmut

AU - Hennig, Bernhard

AU - Toborek, Michal

N1 - Funding Information: This work was supported in part by the Alexander von Humboldt Foundation and by research grants from the Deutsche Forschungsgemeinschaft, American Heart Association, and National Institutes of Health. Publisher Copyright: © 2000 Federation of European Biochemical Societies.

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N2 - The present study was designed to investigate the hypothesis that interleukin-4 (IL-4) can induce apoptosis of human endothelial cells and to study regulatory pathways of this process. Indeed, DNA ladder assay and flow cytometry study showed that IL-4 can induce apoptosis of endothelial cells in a time- and dose-dependent manner. In addition, IL-4 markedly increased activity of caspase-3, and inhibition of this enzyme suppressed IL-4-induced apoptosis in a dose-dependent manner. These results provide the first evidence that IL-4 can induce apoptosis of human endothelial cells. In addition, the data indicate that the caspase-3-dependent pathway is critically involved in this process.

AB - The present study was designed to investigate the hypothesis that interleukin-4 (IL-4) can induce apoptosis of human endothelial cells and to study regulatory pathways of this process. Indeed, DNA ladder assay and flow cytometry study showed that IL-4 can induce apoptosis of endothelial cells in a time- and dose-dependent manner. In addition, IL-4 markedly increased activity of caspase-3, and inhibition of this enzyme suppressed IL-4-induced apoptosis in a dose-dependent manner. These results provide the first evidence that IL-4 can induce apoptosis of human endothelial cells. In addition, the data indicate that the caspase-3-dependent pathway is critically involved in this process.

KW - Apoptosis

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KW - Caspase-3

KW - Human endothelial cell

KW - Interleukin-4

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IL-4 induces apoptosis of endothelial cells through the caspase-3-dependent pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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