The present study was designed to investigate the hypothesis that interleukin-4 (IL-4) can induce apoptosis of human endothelial cells and to study regulatory pathways of this process. Indeed, DNA ladder assay and flow cytometry study showed that IL-4 can induce apoptosis of endothelial cells in a time- and dose-dependent manner. In addition, IL-4 markedly increased activity of caspase-3, and inhibition of this enzyme suppressed IL-4-induced apoptosis in a dose-dependent manner. These results provide the first evidence that IL-4 can induce apoptosis of human endothelial cells. In addition, the data indicate that the caspase-3-dependent pathway is critically involved in this process.
|Number of pages||5|
|State||Published - Nov 24 2000|
Bibliographical noteFunding Information:
This work was supported in part by the Alexander von Humboldt Foundation and by research grants from the Deutsche Forschungsgemeinschaft, American Heart Association, and National Institutes of Health.
© 2000 Federation of European Biochemical Societies.
- Human endothelial cell
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
- Cell Biology