IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study

Camila S. Stolf, Catharina M. Sacramento, Hélvis E.S. Paz, Renato A. Machado, Lucas P. Ramos, Luciane D. de Oliveira, Karina Cogo-Müller, Mauro P. Santamaria, Karina G.S. Ruiz, Renato C.V. Casarin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background and objective: Previous studies have demonstrated an association between the IL10 promoter rs6667202 (C > A) single-nucleotide polymorphism (SNP) and grade C, stage 3 or 4 periodontitis (Perio4C) in the Brazilian population, where the altered A allele was detected more frequently in these patients. However, no functional analysis of this variation has yet been performed. Thus, the objective of this preliminary study was to evaluate the functionality of rs6667202 in gingival fibroblasts (GFs) of individuals with Perio4C and with periodontal health (PH) stimulated with Aggregatibacter actinomycetencomitans protein extract (AaPE). Methods: Patients with PH and Perio4C were segregated according to their genotype (AA, AC, or CC), and a biopsy was performed to establish the culture of the GFs. After GFs exposure to AaPE at 5 µg/ml for 1.5 h, RNA was extracted to analyze IL10 expression by qPCR. Aliquots of the cell's supernatant were subjected to immunoenzymatic analysis (MAGpix) to detect interleukin-10 (IL-10). Results: In PH, the genotypes AA and AC are related to less expression of IL10 (p = 0.027 and p < 0.0001) and less production of IL-10 (p = 0.002 and p = 0.001), when compared to CC. In Perio4C, there was no statistical difference between the genotypes (p > 0.05), although a lower IL-10 expression and release compared with PH CC was seen (p = 0.033 and p < 0.001). Conclusion: The rs6667202 SNP is functional in PH, as it decreases the expression and production of IL-10. In Perio4C, other factors may be masking its action by altering the IL-10's response.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalJournal of Periodontal Research
Volume57
Issue number1
DOIs
StatePublished - Jan 2022

Bibliographical note

Funding Information:
This study was financed by National Council for Scientific and Technological Development—Brazil (CAPES)—Finance Code 001.

Publisher Copyright:
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

ASJC Scopus subject areas

  • Periodontics

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