Illustration of SSMD, z score, SSMD*, z* score, and t statistic for hit selection in RNAi high-throughput screens

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Hit selection is the ultimate goal in many high-throughput screens. Various analytic methods are available for this purpose. Some commonly used ones are z score, z* score, strictly standardized mean difference (SSMD), SSMD*, and t statistic. It is critical to know how to use them correctly because the misusage of them can readily produce misleading results. Here, the author presents basic concepts, elaborates their commonality and difference, describes some common misusage that people should avoid, and uses simulated simple examples to illustrate how to use them correctly.

Original languageEnglish
Pages (from-to)775-785
Number of pages11
JournalJournal of Biomolecular Screening
Volume16
Issue number7
DOIs
StatePublished - Aug 2011

Keywords

  • SSMD
  • SSMD*
  • high-throughput screening
  • t statistic
  • z score
  • z* score

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

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