Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy

Andrew I. Shevchuk; Gregory I. Frolenkov; Daniel Sánchez; Peter S. James; Noah Freedman; Max J. Lab; Roy Jones; David Klenerman; Yuri E. Korchev

doi:10.1002/anie.200503915

Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy

Andrew I. Shevchuk, Gregory I. Frolenkov, Daniel Sánchez, Peter S. James, Noah Freedman, Max J. Lab, Roy Jones, David Klenerman, Yuri E. Korchev

Physiology

Research output: Contribution to journal › Article › peer-review

195 Scopus citations

Abstract

Do not touch! The surface of a living cell is soft and responsive and therefore high-resolution imaging of the cell membrane has not been possible to date. Now noncontact imaging of protein complexes in the plasma membrane of living cells has been demonstrated (see picture) and has been used to follow the cells' structural reorganization. This breakthrough opens up a wealth of new experiments in membrane and cell biology. (Figure Presented)

Original language	English
Pages (from-to)	2212-2216
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	14
DOIs	https://doi.org/10.1002/anie.200503915
State	Published - Mar 27 2006

Keywords

Cells
Imaging
Membrane proteins
Membranes
Scanning probe microscopy

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200503915

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abstract = "Do not touch! The surface of a living cell is soft and responsive and therefore high-resolution imaging of the cell membrane has not been possible to date. Now noncontact imaging of protein complexes in the plasma membrane of living cells has been demonstrated (see picture) and has been used to follow the cells' structural reorganization. This breakthrough opens up a wealth of new experiments in membrane and cell biology. (Figure Presented)",

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AU - Shevchuk, Andrew I.

AU - Frolenkov, Gregory I.

AU - Sánchez, Daniel

AU - James, Peter S.

AU - Freedman, Noah

AU - Lab, Max J.

AU - Jones, Roy

AU - Klenerman, David

AU - Korchev, Yuri E.

PY - 2006/3/27

Y1 - 2006/3/27

N2 - Do not touch! The surface of a living cell is soft and responsive and therefore high-resolution imaging of the cell membrane has not been possible to date. Now noncontact imaging of protein complexes in the plasma membrane of living cells has been demonstrated (see picture) and has been used to follow the cells' structural reorganization. This breakthrough opens up a wealth of new experiments in membrane and cell biology. (Figure Presented)

AB - Do not touch! The surface of a living cell is soft and responsive and therefore high-resolution imaging of the cell membrane has not been possible to date. Now noncontact imaging of protein complexes in the plasma membrane of living cells has been demonstrated (see picture) and has been used to follow the cells' structural reorganization. This breakthrough opens up a wealth of new experiments in membrane and cell biology. (Figure Presented)

KW - Cells

KW - Imaging

KW - Membrane proteins

KW - Membranes

KW - Scanning probe microscopy

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Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy

Abstract

Keywords

ASJC Scopus subject areas

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