Abstract
Immobilizing microalgae cells in a hyphal matrix can simplify harvest while producing novel mycoalgae products with potential food, feed, biomaterial, and renewable energy applications; however, limited quantitative information to describe the process and its applicability under various conditions leads to difficulties in comparing across studies and scaling-up. Here, we demonstrate the immobilization of both active and heat-deactivated marine diatom Phaeodactylum tricornutum (UTEX 466) using different loadings of fungal pellets (Aspergillus sp.) and model the process through kinetics and equilibrium models. Active P. tricornutum cells were not required for the fungal-assisted immobilization process and the fungal isolate was able to immobilize more than its original mass of microalgae. The Freundlich isotherm model adequately described the equilibrium immobilization characteristics and indicated increased normalized algae immobilization (g algae removed/g fungi loaded) under low fungal pellet loadings. The kinetics of algae immobilization by the fungal pellets were found to be adequately modeled using both a pseudo-second order model and a model previously developed for fungal-assisted algae immobilization. These results provide new insights into the behavior and potential applications of fungal-assisted algae immobilization.
Original language | English |
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Pages (from-to) | 251-259 |
Number of pages | 9 |
Journal | Journal of Microbiology and Biotechnology |
Volume | 33 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2023 |
Bibliographical note
Publisher Copyright:© 2023 by the authors.
Funding
Support for this work was provided by the California Energy Commission (ARV-15-008). The authors extend their gratitude to Dr. Zhiliang Fan and Dr. Bryan Jenkins for their review and thoughtful comments on an early version of the manuscript, and Sam Hornstein and Lin Cao for their assistance with lab experiments.
Funders | Funder number |
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California Energy Commission | ARV-15-008 |
California Energy Commission |
Keywords
- Aspergillus sp.
- Freundlich isotherm
- Microalgae immobilization
- Phaeodactylum tricornutum
- fungi
- heat-deactivation
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology