Immunization with the amino-terminus region of dense granule protein 6 (GRA6) of Toxoplasma gondii activates CD8+ cytotoxic T cells capable of removing tissue cysts of the parasite through antigen presentation by human HLA-A2.1

Rajesh Mani, Corinne Mercier, Marie France Delauw, Yasuhiro Suzuki

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

CD8+ T cells from HLA-A2.1-transgenic mice, but not wild-type mice, immunized with the amino-terminus region (aa 41–152) of dense granule protein 6 (GRA6Nt) of Toxoplasma gondii secreted large amounts of perforin and granzyme B in response to GRA6Nt through antigen presentation by HLA-A2.1 in vitro. When those CD8+ T cells were transferred into chronically infected HLA-A2.1-expressing NSG mice deficient in T cells, cerebral cyst burden of the recipients of HLA-A2.1-transgenic T cells, but not of WT T cells, became significantly less than that of control mice with no cell transfer. Furthermore, the significant reduction of the cyst burden by a transfer of the HLA-A2.1-transgenic CD8+ immune T cells required an expression of HLA-A2.1 in the recipient NSG mice. Thus, antigen presentation of GRA6Nt by human HLA-A2.1is able to activate anti-cyst CD8+ T cells that eliminate T. gondii cysts through antigen presentation by human HLA-A2.1.

Original languageEnglish
Article number105182
JournalMicrobes and Infection
Volume25
Issue number8
DOIs
StatePublished - Nov 1 2023

Bibliographical note

Publisher Copyright:
© 2023 Institut Pasteur

Keywords

  • CD8 T cells
  • Dense granule protein 6
  • HLA-A2
  • Protective immunity
  • Tissue cysts
  • Toxoplasma

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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