TY - JOUR
T1 - Immunoaffinity Purification of Human Choline Acetyltransferase
T2 - Comparison of the Brain and Placental Enzymes
AU - Bruce, Gordon
AU - Wainer, Bruce H.
AU - Hersh, Louis B.
PY - 1985/8
Y1 - 1985/8
N2 - Abstract: A rapid and efficient immunoaffinity purification procedure has been developed for human placental choline acetyltransferase (ChAT). Using this procedure, human placental ChAT was purified to homogeneity with high recovery of enzyme activity (50–60%). Purified ChAT was used to raise a monospecific anti‐human ChAT polyclonal antibody in rabbits. A comparison of the physical properties of ChAT was made between the enzymes purified from human brain and human placenta. Only one form of the enzyme exists in either tissue, having identical molecular weights of 68,000 and a single apparent pI of 8.1. A more detailed comparison of the two enzymes using peptide mapping and epitope mapping indicates identity between the brain and placental enzymes.
AB - Abstract: A rapid and efficient immunoaffinity purification procedure has been developed for human placental choline acetyltransferase (ChAT). Using this procedure, human placental ChAT was purified to homogeneity with high recovery of enzyme activity (50–60%). Purified ChAT was used to raise a monospecific anti‐human ChAT polyclonal antibody in rabbits. A comparison of the physical properties of ChAT was made between the enzymes purified from human brain and human placenta. Only one form of the enzyme exists in either tissue, having identical molecular weights of 68,000 and a single apparent pI of 8.1. A more detailed comparison of the two enzymes using peptide mapping and epitope mapping indicates identity between the brain and placental enzymes.
KW - Choline acetyltransferase
KW - Immunoaffinity purification
KW - Polyclonal choline acetyltransferase antibody
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U2 - 10.1111/j.1471-4159.1985.tb04030.x
DO - 10.1111/j.1471-4159.1985.tb04030.x
M3 - Article
C2 - 4009177
AN - SCOPUS:0022363496
SN - 0022-3042
VL - 45
SP - 611
EP - 620
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 2
ER -