Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA

W. E. Woods; C. J. Wang; P. K. Houtz; H. H. Tai; T. Wood; T. J. Weckman; J. M. Yang; S. L. Chang; J. W. Blake; T. Tobin; J. McDonald; S. Kalita; V. D. Bass; P. Weege; B. DeLeon; C. Brockus; S. Wie; R. A. Chung; J. Brecht

Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA

W. E. Woods, C. J. Wang, P. K. Houtz, H. H. Tai, T. Wood, T. J. Weckman, J. M. Yang, S. L. Chang, J. W. Blake, T. Tobin, J. McDonald, S. Kalita, V. D. Bass, P. Weege, B. DeLeon, C. Brockus, S. Wie, R. A. Chung, J. Brecht

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.

Original language	English
Pages (from-to)	111-128
Number of pages	18
Journal	Research Communications in Chemical Pathology and Pharmacology
Volume	61
Issue number	1
State	Published - 1988

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
Pathology and Forensic Medicine
Toxicology
Pharmacology

Cite this

Woods, W. E., Wang, C. J., Houtz, P. K., Tai, H. H., Wood, T., Weckman, T. J., Yang, J. M., Chang, S. L., Blake, J. W., Tobin, T., McDonald, J., Kalita, S., Bass, V. D., Weege, P., DeLeon, B., Brockus, C., Wie, S., Chung, R. A., & Brecht, J. (1988). Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA. Research Communications in Chemical Pathology and Pharmacology, 61(1), 111-128.

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title = "Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA",

abstract = "A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.",

author = "Woods, {W. E.} and Wang, {C. J.} and Houtz, {P. K.} and Tai, {H. H.} and T. Wood and Weckman, {T. J.} and Yang, {J. M.} and Chang, {S. L.} and Blake, {J. W.} and T. Tobin and J. McDonald and S. Kalita and Bass, {V. D.} and P. Weege and B. DeLeon and C. Brockus and S. Wie and Chung, {R. A.} and J. Brecht",

year = "1988",

language = "English",

volume = "61",

pages = "111--128",

number = "1",

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Woods, WE, Wang, CJ, Houtz, PK, Tai, HH, Wood, T, Weckman, TJ, Yang, JM, Chang, SL, Blake, JW, Tobin, T, McDonald, J, Kalita, S, Bass, VD, Weege, P, DeLeon, B, Brockus, C, Wie, S, Chung, RA & Brecht, J 1988, 'Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA', Research Communications in Chemical Pathology and Pharmacology, vol. 61, no. 1, pp. 111-128.

TY - JOUR

T1 - Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA

AU - Woods, W. E.

AU - Wang, C. J.

AU - Houtz, P. K.

AU - Tai, H. H.

AU - Wood, T.

AU - Weckman, T. J.

AU - Yang, J. M.

AU - Chang, S. L.

AU - Blake, J. W.

AU - Tobin, T.

AU - McDonald, J.

AU - Kalita, S.

AU - Bass, V. D.

AU - Weege, P.

AU - DeLeon, B.

AU - Brockus, C.

AU - Wie, S.

AU - Chung, R. A.

AU - Brecht, J.

PY - 1988

Y1 - 1988

N2 - A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.

AB - A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.

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M3 - Article

C2 - 3175339

AN - SCOPUS:0023752599

SN - 0034-5164

VL - 61

SP - 111

EP - 128

JO - Research Communications in Chemical Pathology and Pharmacology

JF - Research Communications in Chemical Pathology and Pharmacology

IS - 1

ER -

Immunoassay detection of drugs in racing horses. VI. Detection of furosemide (Lasix(R)) in equine blood by a one step ELISA and PCFIA

Abstract

ASJC Scopus subject areas

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