Immunocytochemical method for investigating in vivo neuronal oxygen radical-induced lipid peroxidation

Edward D. Hall, Jo A. Oostveen, Paula K. Andrus, Douglas K. Anderson, Craig E. Thomas

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The investigation of oxygen radical-induced lipid peroxidative neuronal damage in the context of acute and chronic neurodegenerative disorders has been largely limited to the use of ex vivo analytical methodologies. These are often fraught with sensitivity or specificity problems, or they are indirect. Furthermore, none of the analytical methods allow precise anatomical identification of the cells that are undergoing peroxidative injury. This paper describes an immunocytochemical method for localization of central nervous system (CNS) lipid peroxidation (LP) that employs a rabbit-derived antibody raised against malondialdehyde (MDA)- modified rabbit serum albumin (RSA). MDA is a breakdown product of peroxidized membrane polyunsaturated fatty acids that avidly binds to cellular proteins. Using the anti-MDA-RSA, we herein illustrate increased MDA-derived immunostaining: (1) in the spinal cord of transgenic familial amyotrophic lateral sclerosis (ALS) mice; and (2) in the selectively vulnerable gerbil hippocampal CA1 region after a 5 min episode of forebrain ischemia and its relationship to the time course of neuronal degeneration.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalJournal of Neuroscience Methods
Volume76
Issue number2
DOIs
StatePublished - Oct 3 1997

Keywords

  • Amyotrophic lateral sclerosis
  • Cerebral ischemia
  • Immunocytochemistry
  • Lipid peroxidation
  • Malondialdehyde

ASJC Scopus subject areas

  • Neuroscience (all)

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