Immunohistochemical localization of Mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein

J. M. Hiller; Y. Zhang; G. Bing; T. L. Gioannini; E. A. Stone; E. J. Simon

doi:10.1016/0306-4522(94)90480-4

Immunohistochemical localization of Mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein

J. M. Hiller, Y. Zhang, G. Bing, T. L. Gioannini, E. A. Stone, E. J. Simon

Neuroscience

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Abstract

Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the μ-opioid binding protein purified in our laboratory. Immunohistochemical staining with Ab165 was carried out by the avidin-biotin procedure. Antibody, preabsorbed with antigen, served as control. Extensive immunoreactivity was seen in the hippocampal formation, the amygdaloid complex, the striatal complex, cortical regions, select areas of the thalamus and hypothalamus and in laminae I and II of the dorsal horn in spinal cord. The distribution of immunoreactivity in the rat brain of antibody 165, which recognizes a purified μ-opioid binding protein, is concordant with the distribution of μ-opioid binding sites as determined by other laboratories in autoradiographic, electrophysiological and immunocytochemical studies. These findings have enabled us to distinguish areas possessing large fields of μ-opioid receptor containing cell bodies from areas possessing dense networks of immunolabeled neuronal processes or mixtures of both.

Original language	English
Pages (from-to)	829-841
Number of pages	13
Journal	Neuroscience
Volume	62
Issue number	3
DOIs	https://doi.org/10.1016/0306-4522(94)90480-4
State	Published - Oct 1994

Bibliographical note

Funding Information:
The immunohistochemiclaolc alizationp atterni n rat CNS of p-opioid receptortsh atw e haveo bserved here,u singA bl65, is concordantw ith the mapping previously obtained by autoradiographicm eans. The findingsp resenteedx pando ur informationt o the levelo f distinguishingb etweena reasp ossessinlga rge fieldso f p-opioid receptocr ontainingc ell bodiesa nd DA00017fr omthe National Institute on DFU~ Abuse to Ar,~noil%e~geement..-.Twhjosr k was s~p~rtedb y Grant areasp ossessingd ensen etworkso f itnmunolabeled EJS and Grants MH45265 andA FOSR F4%2~92-J-~84 neuronalp rocesseosr mixtureso f both. Use of the to EM.

Funding

The immunohistochemiclaolc alizationp atterni n rat CNS of p-opioid receptortsh atw e haveo bserved here,u singA bl65, is concordantw ith the mapping previously obtained by autoradiographicm eans. The findingsp resenteedx pando ur informationt o the levelo f distinguishingb etweena reasp ossessinlga rge fieldso f p-opioid receptocr ontainingc ell bodiesa nd DA00017fr omthe National Institute on DFU~ Abuse to Ar,~noil%e~geement..-.Twhjosr k was s~p~rtedb y Grant areasp ossessingd ensen etworkso f itnmunolabeled EJS and Grants MH45265 andA FOSR F4%2~92-J-~84 neuronalp rocesseosr mixtureso f both. Use of the to EM.

Funders	Funder number
National Institute of Mental Health	R01MH045265

ASJC Scopus subject areas

General Neuroscience

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10.1016/0306-4522(94)90480-4

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@article{4841ae8ec3cf47d4bab25bccc489196d,

title = "Immunohistochemical localization of Mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein",

abstract = "Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the μ-opioid binding protein purified in our laboratory. Immunohistochemical staining with Ab165 was carried out by the avidin-biotin procedure. Antibody, preabsorbed with antigen, served as control. Extensive immunoreactivity was seen in the hippocampal formation, the amygdaloid complex, the striatal complex, cortical regions, select areas of the thalamus and hypothalamus and in laminae I and II of the dorsal horn in spinal cord. The distribution of immunoreactivity in the rat brain of antibody 165, which recognizes a purified μ-opioid binding protein, is concordant with the distribution of μ-opioid binding sites as determined by other laboratories in autoradiographic, electrophysiological and immunocytochemical studies. These findings have enabled us to distinguish areas possessing large fields of μ-opioid receptor containing cell bodies from areas possessing dense networks of immunolabeled neuronal processes or mixtures of both.",

author = "Hiller, {J. M.} and Y. Zhang and G. Bing and Gioannini, {T. L.} and Stone, {E. A.} and Simon, {E. J.}",

note = "Funding Information: The immunohistochemiclaolc alizationp atterni n rat CNS of p-opioid receptortsh atw e haveo bserved here,u singA bl65, is concordantw ith the mapping previously obtained by autoradiographicm eans. The findingsp resenteedx pando ur informationt o the levelo f distinguishingb etweena reasp ossessinlga rge fieldso f p-opioid receptocr ontainingc ell bodiesa nd DA00017fr omthe National Institute on DFU~ Abuse to Ar,~noil%e~geement..-.Twhjosr k was s~p~rtedb y Grant areasp ossessingd ensen etworkso f itnmunolabeled EJS and Grants MH45265 andA FOSR F4%2~92-J-~84 neuronalp rocesseosr mixtureso f both. Use of the to EM.",

year = "1994",

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doi = "10.1016/0306-4522(94)90480-4",

language = "English",

volume = "62",

pages = "829--841",

number = "3",

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T1 - Immunohistochemical localization of Mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein

AU - Hiller, J. M.

AU - Zhang, Y.

AU - Bing, G.

AU - Gioannini, T. L.

AU - Stone, E. A.

AU - Simon, E. J.

N1 - Funding Information: The immunohistochemiclaolc alizationp atterni n rat CNS of p-opioid receptortsh atw e haveo bserved here,u singA bl65, is concordantw ith the mapping previously obtained by autoradiographicm eans. The findingsp resenteedx pando ur informationt o the levelo f distinguishingb etweena reasp ossessinlga rge fieldso f p-opioid receptocr ontainingc ell bodiesa nd DA00017fr omthe National Institute on DFU~ Abuse to Ar,~noil%e~geement..-.Twhjosr k was s~p~rtedb y Grant areasp ossessingd ensen etworkso f itnmunolabeled EJS and Grants MH45265 andA FOSR F4%2~92-J-~84 neuronalp rocesseosr mixtureso f both. Use of the to EM.

PY - 1994/10

Y1 - 1994/10

N2 - Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the μ-opioid binding protein purified in our laboratory. Immunohistochemical staining with Ab165 was carried out by the avidin-biotin procedure. Antibody, preabsorbed with antigen, served as control. Extensive immunoreactivity was seen in the hippocampal formation, the amygdaloid complex, the striatal complex, cortical regions, select areas of the thalamus and hypothalamus and in laminae I and II of the dorsal horn in spinal cord. The distribution of immunoreactivity in the rat brain of antibody 165, which recognizes a purified μ-opioid binding protein, is concordant with the distribution of μ-opioid binding sites as determined by other laboratories in autoradiographic, electrophysiological and immunocytochemical studies. These findings have enabled us to distinguish areas possessing large fields of μ-opioid receptor containing cell bodies from areas possessing dense networks of immunolabeled neuronal processes or mixtures of both.

AB - Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the μ-opioid binding protein purified in our laboratory. Immunohistochemical staining with Ab165 was carried out by the avidin-biotin procedure. Antibody, preabsorbed with antigen, served as control. Extensive immunoreactivity was seen in the hippocampal formation, the amygdaloid complex, the striatal complex, cortical regions, select areas of the thalamus and hypothalamus and in laminae I and II of the dorsal horn in spinal cord. The distribution of immunoreactivity in the rat brain of antibody 165, which recognizes a purified μ-opioid binding protein, is concordant with the distribution of μ-opioid binding sites as determined by other laboratories in autoradiographic, electrophysiological and immunocytochemical studies. These findings have enabled us to distinguish areas possessing large fields of μ-opioid receptor containing cell bodies from areas possessing dense networks of immunolabeled neuronal processes or mixtures of both.

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AN - SCOPUS:0028171967

SN - 0306-4522

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EP - 841

JO - Neuroscience

JF - Neuroscience

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ER -

Immunohistochemical localization of Mu-opioid receptors in rat brain using antibodies generated against a peptide sequence present in a purified mu-opioid binding protein

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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