Light-microscope visualization in rat brain of a pattern of distribution of immunoreactivity, which included immunolabeled perikarya and beaded processes, was achieved using an immunoaffinity purified polyclonal antibody, Ab165, which recognizes the amino acid sequence, IRNLRQDRSKYY, found in the μ-opioid binding protein purified in our laboratory. Immunohistochemical staining with Ab165 was carried out by the avidin-biotin procedure. Antibody, preabsorbed with antigen, served as control. Extensive immunoreactivity was seen in the hippocampal formation, the amygdaloid complex, the striatal complex, cortical regions, select areas of the thalamus and hypothalamus and in laminae I and II of the dorsal horn in spinal cord. The distribution of immunoreactivity in the rat brain of antibody 165, which recognizes a purified μ-opioid binding protein, is concordant with the distribution of μ-opioid binding sites as determined by other laboratories in autoradiographic, electrophysiological and immunocytochemical studies. These findings have enabled us to distinguish areas possessing large fields of μ-opioid receptor containing cell bodies from areas possessing dense networks of immunolabeled neuronal processes or mixtures of both.
|Number of pages
|Published - Oct 1994
Bibliographical noteFunding Information:
The immunohistochemiclaolc alizationp atterni n rat CNS of p-opioid receptortsh atw e haveo bserved here,u singA bl65, is concordantw ith the mapping previously obtained by autoradiographicm eans. The findingsp resenteedx pando ur informationt o the levelo f distinguishingb etweena reasp ossessinlga rge fieldso f p-opioid receptocr ontainingc ell bodiesa nd DA00017fr omthe National Institute on DFU~ Abuse to Ar,~noil%e~geement..-.Twhjosr k was s~p~rtedb y Grant areasp ossessingd ensen etworkso f itnmunolabeled EJS and Grants MH45265 andA FOSR F4%2~92-J-~84 neuronalp rocesseosr mixtureso f both. Use of the to EM.
ASJC Scopus subject areas
- Neuroscience (all)