Immunolocalization of anti-Müllerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares

Tsogtgerel Munkhtuul, Harutaka Murase, Barry A. Ball, Kayo Habukawa, Fumio Sato, Kenichi Watanabe, Yasuo Nambo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalJournal of Equine Veterinary Science
StatePublished - Mar 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Inc.


  • Anti-Müllerian hormone
  • Anti-Müllerian hormone receptor type 2
  • Granulosa cell tumor
  • Immunohistochemistry
  • Mare

ASJC Scopus subject areas

  • Equine


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