Immunolocalization of anti-Müllerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares

Tsogtgerel Munkhtuul; Harutaka Murase; Barry A. Ball; Kayo Habukawa; Fumio Sato; Kenichi Watanabe; Yasuo Nambo

doi:10.1016/j.jevs.2018.12.012

Immunolocalization of anti-Müllerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares

Tsogtgerel Munkhtuul, Harutaka Murase, Barry A. Ball, Kayo Habukawa, Fumio Sato, Kenichi Watanabe, Yasuo Nambo

Veterinary Science

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.

Original language	English
Pages (from-to)	9-12
Number of pages	4
Journal	Journal of Equine Veterinary Science
Volume	74
DOIs	https://doi.org/10.1016/j.jevs.2018.12.012
State	Published - Mar 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

Anti-Müllerian hormone
Anti-Müllerian hormone receptor type 2
Granulosa cell tumor
Immunohistochemistry
Mare

ASJC Scopus subject areas

Equine

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10.1016/j.jevs.2018.12.012

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title = "Immunolocalization of anti-M{\"u}llerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares",

abstract = "Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-M{\"u}llerian hormone (AMH) and its receptor, anti-M{\"u}llerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.",

keywords = "Anti-M{\"u}llerian hormone, Anti-M{\"u}llerian hormone receptor type 2, Granulosa cell tumor, Immunohistochemistry, Mare",

author = "Tsogtgerel Munkhtuul and Harutaka Murase and Ball, {Barry A.} and Kayo Habukawa and Fumio Sato and Kenichi Watanabe and Yasuo Nambo",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2019",

month = mar,

doi = "10.1016/j.jevs.2018.12.012",

language = "English",

volume = "74",

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AU - Munkhtuul, Tsogtgerel

AU - Murase, Harutaka

AU - Ball, Barry A.

AU - Habukawa, Kayo

AU - Sato, Fumio

AU - Watanabe, Kenichi

AU - Nambo, Yasuo

PY - 2019/3

Y1 - 2019/3

N2 - Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.

AB - Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.

KW - Anti-Müllerian hormone

KW - Anti-Müllerian hormone receptor type 2

KW - Granulosa cell tumor

KW - Immunohistochemistry

KW - Mare

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Immunolocalization of anti-Müllerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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