TY - JOUR
T1 - Immunolocalization of anti-Müllerian Hormone and Its Receptor in Granulosa Cell Tumors in Mares
AU - Munkhtuul, Tsogtgerel
AU - Murase, Harutaka
AU - Ball, Barry A.
AU - Habukawa, Kayo
AU - Sato, Fumio
AU - Watanabe, Kenichi
AU - Nambo, Yasuo
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/3
Y1 - 2019/3
N2 - Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.
AB - Granulosa cell tumor (GCT) is a sex-cord stromal tumor in mares and causes infertility. The objective of this study was to localize anti-Müllerian hormone (AMH) and its receptor, anti-Müllerian hormone receptor type 2 (AMHR2), in ovarian tissue samples obtained from 11 Thoroughbred mares diagnosed with GCT. Immunohistochemistry revealed positive immunostaining for both AMH and AMHR2 in the granulosa-like cells of GCT. Furthermore, double immunofluorescence staining revealed the presence of colocalization of AMH and AMHR2 in granulosa-like cells of GCT in mares. These findings suggest that granulosa-like cells of GCT are a target of AMH, suggesting that AMH may have paracrine and autocrine function on granulosa-like cells of GCT in mares. Moreover, this is the first report to demonstrate the colocalization of AMH and its receptor, AMHR2, in GCT-affected ovaries of mares by using double immunofluorescence staining.
KW - Anti-Müllerian hormone
KW - Anti-Müllerian hormone receptor type 2
KW - Granulosa cell tumor
KW - Immunohistochemistry
KW - Mare
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U2 - 10.1016/j.jevs.2018.12.012
DO - 10.1016/j.jevs.2018.12.012
M3 - Article
AN - SCOPUS:85059704397
SN - 0737-0806
VL - 74
SP - 9
EP - 12
JO - Journal of Equine Veterinary Science
JF - Journal of Equine Veterinary Science
ER -