Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats

Tábata De Mello Tera, Rodrigo Dias Nascimento, Renata Falchete Do Prado, Mauro Pedrine Santamaria, Maria Aparecida Neves Jardini

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Objective: The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods: Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results: All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions: The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.

Original languageEnglish
Pages (from-to)541-553
Number of pages13
JournalJournal of Applied Oral Science
Issue number6
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© 2014, Faculdade de Odontologia de Bauru. All rights reserved.


  • Bone regeneration
  • Estrogen
  • Osteocalcin
  • Osteonectin. Sialoprotein

ASJC Scopus subject areas

  • General Dentistry


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