TY - JOUR
T1 - Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults
AU - Terrando, Niccolò
AU - Park, John J.
AU - Devinney, Michael
AU - Chan, Cliburn
AU - Cooter, Mary
AU - Avasarala, Pallavi
AU - Mathew, Joseph P.
AU - Quinones, Quintin J.
AU - Maddipati, Krishna Rao
AU - Berger, Miles
AU - Brigman, Brian
AU - Browndyke, Jeffrey
AU - Bullock, William M.
AU - Carter, Jessica
AU - Chapman, Joseph
AU - Colin, Brian
AU - D’Amico, Thomas A.
AU - DeOrio, James K.
AU - Esclamado, Ramon M.
AU - Ferrandino, Michael N.
AU - Gadsden, Jeffrey
AU - Garrigues, Grant E.
AU - Guercio, Jason
AU - Habib, Ashraf
AU - Harpole, David H.
AU - Hartwig, Mathew G.
AU - Iboaya, Ehimemen
AU - Inman, Brant A.
AU - Khan, Anver
AU - Lagoo-Deenadayalan, Sandhya
AU - Lee, Paula S.
AU - Lee, Walter T.
AU - Lemm, John
AU - Levinson, Howard
AU - Mantyh, Christopher
AU - McDonagh, David L.
AU - Migaly, John
AU - Mithani, Suhail K.
AU - Moretti, Eugene
AU - Moul, Judd W.
AU - Newman, Mark F.
AU - Ohlendorf, Brian
AU - Perez, Alexander
AU - Peterson, Andrew C.
AU - Preminger, Glenn M.
AU - Robertson, Cary N.
AU - Roman, Sanziana A.
AU - Runyon, Scott
AU - Sandler, Aaron
AU - Sbahi, Faris M.
N1 - Funding Information:
This work was supported by Duke CTSA grant (UL1TR001117) as well as Duke Anesthesiology departmental funds, and a mentored research grant from the International Anesthesia Research Society. Dr Berger also acknowledges additional support from National Institutes of Health (NIH) grants T32 GM08600, R03AG050918, 1K76AG057022, and the Duke Claude D. Pepper Older American Independence Center (P30AG028716), and a William L. Young neuroscience research award from the Society for Neuroscience in Anesthesiology and Critical Care (SNACC). KM also acknowledges support from the National Center for Research Resources, NIH Grant S10RR027926.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography–mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.
AB - Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography–mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.
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U2 - 10.1038/s41598-021-82606-5
DO - 10.1038/s41598-021-82606-5
M3 - Article
C2 - 33542362
AN - SCOPUS:85100489342
VL - 11
IS - 1
M1 - 3047
ER -