Impact of an SGLT2-loss of function mutation on renal architecture, histology, and glucose homeostasis

Corey B. Hughes; George M. Mussman; Phil Ray; Robert C. Bunn; Virgilius Cornea; Kathryn M. Thrailkill; John L. Fowlkes; Iuliana Popescu

doi:10.1007/s00441-020-03358-8

Impact of an SGLT2-loss of function mutation on renal architecture, histology, and glucose homeostasis

Corey B. Hughes, George M. Mussman, Phil Ray, Robert C. Bunn, Virgilius Cornea, Kathryn M. Thrailkill, John L. Fowlkes, Iuliana Popescu

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Inhibitors of sodium/glucose co-transporter 2 (SGLT2) are currently in clinical use for type 2 diabetes (T2D) treatment due to their anti-hyperglycemic effect exerted by the inhibition of glucose reabsorption in the kidney. Inhibition of SGLT2 is associated with improvement of renal outcomes in chronic kidney disease associated with T2D. Our study aimed to describe the renal-specific phenotypic consequences of the SGLT2-loss of function “Jimbee” mutation within the Slc5a2 mouse gene in a non-diabetic/non-obese background. The Jimbee mice displayed reduced body weight, glucosuria, polyuria, polydipsia, and hyperphagia but were normoglycemic, with no signs of baseline insulin resistance or renal dysfunction. Histomorphological analysis of the kidneys revealed a normal architecture and morphology of the renal cortex, but shrinkage of the glomerular and tubular apparatus, including Bowman’s space, glomerular tuft, mesangial matrix fraction, and proximal convoluted tubule (PCT). Immunofluorescent analysis of renal sections showed that SGLT2 was absent from the apical membrane of PCT of the Jimbee mice but remnant positive vesicles were detected within the cytosol or at the perinuclear interface. Renal localization and abundance of GLUT1, GLUT2, and SGLT1 were unchanged in the Jimbee genotype. Intriguingly, the mutation did not induce hepatic gluconeogenic gene expression in overnight fasted mice despite a high glucose excretion rate. The Jimbee phenotype is remarkably similar to humans with SLC5A2 mutations and provides a useful model for the study of SGLT2-loss of function effects on renal architecture and physiology, as well as for identifying possible novel roles for the kidneys in glucose homeostasis and metabolic reprogramming.

Original language	English
Pages (from-to)	527-543
Number of pages	17
Journal	Cell and Tissue Research
Volume	384
Issue number	2
DOIs	https://doi.org/10.1007/s00441-020-03358-8
State	Published - May 2021

Bibliographical note

Funding Information:
We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee ” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Funding Information:
This work was supported by grants from the National Institutes of Health, R21AR070620 (to K.M.T.) and R56DK084045 (to J.L.F.). Additional funding was provided by the University of Kentucky Barnstable Brown Diabetes Center Research Endowment. C.B.H and G.M.M were students enrolled in the HON385/ BIO395, respectively BIO395 Mentored Research Programs of the University of Kentucky under the mentorship of I.P.

Funding Information:
We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Publisher Copyright:
© 2021, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Gluconeogenesis
Kidney
Mutation
Phenotype
SGLT2

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00441-020-03358-8

Cite this

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title = "Impact of an SGLT2-loss of function mutation on renal architecture, histology, and glucose homeostasis",

abstract = "Inhibitors of sodium/glucose co-transporter 2 (SGLT2) are currently in clinical use for type 2 diabetes (T2D) treatment due to their anti-hyperglycemic effect exerted by the inhibition of glucose reabsorption in the kidney. Inhibition of SGLT2 is associated with improvement of renal outcomes in chronic kidney disease associated with T2D. Our study aimed to describe the renal-specific phenotypic consequences of the SGLT2-loss of function “Jimbee” mutation within the Slc5a2 mouse gene in a non-diabetic/non-obese background. The Jimbee mice displayed reduced body weight, glucosuria, polyuria, polydipsia, and hyperphagia but were normoglycemic, with no signs of baseline insulin resistance or renal dysfunction. Histomorphological analysis of the kidneys revealed a normal architecture and morphology of the renal cortex, but shrinkage of the glomerular and tubular apparatus, including Bowman{\textquoteright}s space, glomerular tuft, mesangial matrix fraction, and proximal convoluted tubule (PCT). Immunofluorescent analysis of renal sections showed that SGLT2 was absent from the apical membrane of PCT of the Jimbee mice but remnant positive vesicles were detected within the cytosol or at the perinuclear interface. Renal localization and abundance of GLUT1, GLUT2, and SGLT1 were unchanged in the Jimbee genotype. Intriguingly, the mutation did not induce hepatic gluconeogenic gene expression in overnight fasted mice despite a high glucose excretion rate. The Jimbee phenotype is remarkably similar to humans with SLC5A2 mutations and provides a useful model for the study of SGLT2-loss of function effects on renal architecture and physiology, as well as for identifying possible novel roles for the kidneys in glucose homeostasis and metabolic reprogramming.",

keywords = "Gluconeogenesis, Kidney, Mutation, Phenotype, SGLT2",

author = "Hughes, {Corey B.} and Mussman, {George M.} and Phil Ray and Bunn, {Robert C.} and Virgilius Cornea and Thrailkill, {Kathryn M.} and Fowlkes, {John L.} and Iuliana Popescu",

note = "Funding Information: We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee ” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center. Funding Information: This work was supported by grants from the National Institutes of Health, R21AR070620 (to K.M.T.) and R56DK084045 (to J.L.F.). Additional funding was provided by the University of Kentucky Barnstable Brown Diabetes Center Research Endowment. C.B.H and G.M.M were students enrolled in the HON385/ BIO395, respectively BIO395 Mentored Research Programs of the University of Kentucky under the mentorship of I.P. Funding Information: We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center. Publisher Copyright: {\textcopyright} 2021, Springer-Verlag GmbH Germany, part of Springer Nature.",

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doi = "10.1007/s00441-020-03358-8",

language = "English",

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AU - Mussman, George M.

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AU - Bunn, Robert C.

AU - Cornea, Virgilius

AU - Thrailkill, Kathryn M.

AU - Fowlkes, John L.

AU - Popescu, Iuliana

N1 - Funding Information: We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee ” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center. Funding Information: This work was supported by grants from the National Institutes of Health, R21AR070620 (to K.M.T.) and R56DK084045 (to J.L.F.). Additional funding was provided by the University of Kentucky Barnstable Brown Diabetes Center Research Endowment. C.B.H and G.M.M were students enrolled in the HON385/ BIO395, respectively BIO395 Mentored Research Programs of the University of Kentucky under the mentorship of I.P. Funding Information: We thank Dr. Thomas Wilkop, from the Light Microscopy Core at the University of Kentucky, Lexington, KY, for imaging services and valuable advice regarding the SIM experiments. This research was supported by the Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558). The mouse strain used for this research project, C57BL/6J-Slc5a2m1Btlr/Mmmh, RRID: MMRRC_036517-MU, (the “Jimbee” strain) was obtained from the Mutant Mouse Resource and Research Center (MMRRC) at University of Missouri, an NIH-funded strain repository, and was donated to the MMRRC by Bruce Beutler, M.D., University of Texas Southwestern Medical Center. Publisher Copyright: © 2021, Springer-Verlag GmbH Germany, part of Springer Nature.

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N2 - Inhibitors of sodium/glucose co-transporter 2 (SGLT2) are currently in clinical use for type 2 diabetes (T2D) treatment due to their anti-hyperglycemic effect exerted by the inhibition of glucose reabsorption in the kidney. Inhibition of SGLT2 is associated with improvement of renal outcomes in chronic kidney disease associated with T2D. Our study aimed to describe the renal-specific phenotypic consequences of the SGLT2-loss of function “Jimbee” mutation within the Slc5a2 mouse gene in a non-diabetic/non-obese background. The Jimbee mice displayed reduced body weight, glucosuria, polyuria, polydipsia, and hyperphagia but were normoglycemic, with no signs of baseline insulin resistance or renal dysfunction. Histomorphological analysis of the kidneys revealed a normal architecture and morphology of the renal cortex, but shrinkage of the glomerular and tubular apparatus, including Bowman’s space, glomerular tuft, mesangial matrix fraction, and proximal convoluted tubule (PCT). Immunofluorescent analysis of renal sections showed that SGLT2 was absent from the apical membrane of PCT of the Jimbee mice but remnant positive vesicles were detected within the cytosol or at the perinuclear interface. Renal localization and abundance of GLUT1, GLUT2, and SGLT1 were unchanged in the Jimbee genotype. Intriguingly, the mutation did not induce hepatic gluconeogenic gene expression in overnight fasted mice despite a high glucose excretion rate. The Jimbee phenotype is remarkably similar to humans with SLC5A2 mutations and provides a useful model for the study of SGLT2-loss of function effects on renal architecture and physiology, as well as for identifying possible novel roles for the kidneys in glucose homeostasis and metabolic reprogramming.

AB - Inhibitors of sodium/glucose co-transporter 2 (SGLT2) are currently in clinical use for type 2 diabetes (T2D) treatment due to their anti-hyperglycemic effect exerted by the inhibition of glucose reabsorption in the kidney. Inhibition of SGLT2 is associated with improvement of renal outcomes in chronic kidney disease associated with T2D. Our study aimed to describe the renal-specific phenotypic consequences of the SGLT2-loss of function “Jimbee” mutation within the Slc5a2 mouse gene in a non-diabetic/non-obese background. The Jimbee mice displayed reduced body weight, glucosuria, polyuria, polydipsia, and hyperphagia but were normoglycemic, with no signs of baseline insulin resistance or renal dysfunction. Histomorphological analysis of the kidneys revealed a normal architecture and morphology of the renal cortex, but shrinkage of the glomerular and tubular apparatus, including Bowman’s space, glomerular tuft, mesangial matrix fraction, and proximal convoluted tubule (PCT). Immunofluorescent analysis of renal sections showed that SGLT2 was absent from the apical membrane of PCT of the Jimbee mice but remnant positive vesicles were detected within the cytosol or at the perinuclear interface. Renal localization and abundance of GLUT1, GLUT2, and SGLT1 were unchanged in the Jimbee genotype. Intriguingly, the mutation did not induce hepatic gluconeogenic gene expression in overnight fasted mice despite a high glucose excretion rate. The Jimbee phenotype is remarkably similar to humans with SLC5A2 mutations and provides a useful model for the study of SGLT2-loss of function effects on renal architecture and physiology, as well as for identifying possible novel roles for the kidneys in glucose homeostasis and metabolic reprogramming.

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Impact of an SGLT2-loss of function mutation on renal architecture, histology, and glucose homeostasis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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