Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Yueh Fen Li; Jian Shi; Michael C. Nelson; Po Hsu Chen; Joerg Graf; Yebo Li; Zhongtang Yu

doi:10.1016/j.biortech.2015.10.078

Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Yueh Fen Li
, Jian Shi
, Michael C. Nelson
, Po Hsu Chen
, Joerg Graf
, Yebo Li
, Zhongtang Yu

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

Original language	English
Pages (from-to)	744-752
Number of pages	9
Journal	Bioresource Technology
Volume	200
DOIs	https://doi.org/10.1016/j.biortech.2015.10.078
State	Published - Jan 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Funding

This study was partially supported by a Department of Energy (DOE) award ( DE-FG36-05GO85010 ) and a United States Department of Agriculture/ National Institute of Food and Agriculture (USDA/NIFA) award ( 2012-10008-20302 ).

Funders	Funder number
U.S. Department of Energy EPSCoR	DE-FG36-05GO85010
U.S. Department of Energy EPSCoR
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative	2012-10008-20302
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

Keywords

16S rRNA gene
Illumina sequencing
Metagenomics
Microbiome
Succession

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2015.10.078

Cite this

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abstract = "The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.",

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doi = "10.1016/j.biortech.2015.10.078",

language = "English",

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AU - Li, Yueh Fen

AU - Shi, Jian

AU - Nelson, Michael C.

AU - Chen, Po Hsu

AU - Graf, Joerg

AU - Li, Yebo

AU - Yu, Zhongtang

PY - 2016/1/1

Y1 - 2016/1/1

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AB - The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

KW - 16S rRNA gene

KW - Illumina sequencing

KW - Metagenomics

KW - Microbiome

KW - Succession

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Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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