Impact of individual acute phase serum amyloid a isoforms on HDL metabolism in mice

Myung Hee Kim, Maria C. De Beer, Joanne M. Wroblewski, Richard J. Charnigo, Ailing Ji, Nancy R. Webb, Frederick C. De Beer, Deneys R. Van Der Westhuyzen

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The acute phase (AP) reactant serum amyloid A (SAA), an HDL apolipoprotein, exhibits pro-inflammatory activities, but its physiological function(s) are poorly understood. Functional differences between SAA1.1 and SAA2.1, the two major SAA isoforms, are unclear. Mice deficient in either isoform were used to investigate plasma isoform effects on HDL structure, composition, and apolipoprotein catabolism. Lack of either isoform did not affect the size of HDL, normally enlarged in the AP, and did not significantly change HDL composition. Plasma clearance rates of HDL apolipoproteins were determined using native HDL particles. The fractional clearance rates (FCRs) of apoA-I, apoAII, and SAA were distinct, indicating that HDL is not cleared as intact particles. The FCRs of SAA1.1 and SAA2.1 in AP mice were similar, suggesting that the selective deposition of SAA1.1 in amyloid plaques is not associated with a difference in the rates of plasma clearance of the isoforms. Although the clearance rate of SAA was reduced in the absence of the HDL receptor, scavenger receptor class B type I (SRBI), it remained significantly faster compared with that of apoA-I and apoA-II, indicating a relatively minor role of SRBI in SAA's rapid clearance. These studies enhance our understanding of SAA metabolism and SAA's effects on AP-HDL composition and catabolism.-Kim, M-H., M. C. de Beer, J. M. Wroblewski, R. J. Charnigo, A. Ji, N. R. Webb, F. C. de Beer, and D. R. van der Westhuyzen. Impact of individual acute phase serum amyloid A isoforms on HDL metabolism in mice.

Original languageEnglish
Pages (from-to)969-979
Number of pages11
JournalJournal of Lipid Research
Volume57
Issue number6
DOIs
StatePublished - Jun 2016

Bibliographical note

Publisher Copyright:
Copyright © 2016 by the American Society for Biochemistry and Molecular Biology, Inc.

Keywords

  • Acute phase protein
  • Apolipoproteins
  • High density lipoprotein
  • Inflammation
  • Lipoproteins/metabolism
  • Scavenger receptor class B type 1
  • Scavenger receptors

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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