Impaired antigen receptor induced calcium mobilization in a phospholipase C-γ1 deficient B cell line

Natarajan Muthusamy, Do Joon Park, Hei Won Rho, Sue Goo Rhee, Bondada Subbarao

Research output: Contribution to journalArticlepeer-review

Abstract

B lymphocytes express phospholipase C-γ1 (PLC-γ 1) and phospholipase C-γ2 (PLC-γ2) isozymes. However, the relative importance of these two isozymes in B cell signaling is not known. We report here the identification and analysis of a B cell line deficient in PLC-γ1. Mature splenic B lymphocytes and a panel of cell lines representing pre-B, immature and mature B cell stages expressed phospholipase C-γ (PLC-γ), but not the β or δ isoforms of phospholipase C (PLC). While all the tested B cell lines and primary splenic B cells expressed PLC-γ1 and PLC-γ 2 isozymes, the L1.2 B cell line exclusively expressed PLC-γ2, but not PLC-γ1 isozyme. The PLC-γ1 deficient L1.2 B cells expressed levels of surface IgM comparable to that of PLC-γ1 positive 70Z/3 B cell line. However, stimulation through the antigen receptor induced Ca++ response in 70Z/3, but not L1.2 B cells, suggesting a requirement for PLC-γ1 in antigen receptor induced Ca++ signaling in these cells. The possible use for L1.2 cells in testing the regulation of PLC-γ1 and PLC-γ2 dependent calcium mobilization in B lymphocytes is discussed.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalImmunology Letters
Volume90
Issue number2-3
DOIs
StatePublished - Dec 15 2003

Keywords

  • Antigen receptor
  • B lymphocytes
  • Calcium signaling
  • Phospholipase C isozymes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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