Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats

David Kidwell; Sriram Subramaniam; Cobern Ott; Brian Jackson

doi:10.1016/0014-2999(90)94186-2

Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats

David Kidwell, Sriram Subramaniam, Cobern Ott, Brian Jackson

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4 Scopus citations

Abstract

We have examined the effects of chronic gentamicin treatment on arginine⁸-vasopressin (AVP)-dependent cyclic AMP (cAMP) metabolism in rat medullary collecting tubules (oMCT) and medullary thick ascending limbs of Henle's loop (mTALH). Gentamicin attenuated AVP-stimulated cAMP accumulation to a greater extent in the mTALH (Δ -51%) than in the oMCT (Δ -25%). The mechanism of attenuation differed between segments, and could not be attributed to either direct inhibition of adenylate cyclase activity nor direct potentiation of cAMP-phosphodiesterase activity. These data suggest that the gentamicin-induced decrease in renal concentrating ability may be due at least in part to reduced AVP-dependent cAMP accumulation in the oMCT and mTALH.

Original language	English
Pages (from-to)	489-492
Number of pages	4
Journal	European Journal of Pharmacology
Volume	191
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(90)94186-2
State	Published - Dec 4 1990

Keywords

(thick ascending limb of)
5′-cyclic monophosphate
AVP
Adenosine 3′
Aminogiycosides
Collecting tubule
Henle's loop
[Arg]vasopressin

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(90)94186-2

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title = "Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats",

abstract = "We have examined the effects of chronic gentamicin treatment on arginine8-vasopressin (AVP)-dependent cyclic AMP (cAMP) metabolism in rat medullary collecting tubules (oMCT) and medullary thick ascending limbs of Henle's loop (mTALH). Gentamicin attenuated AVP-stimulated cAMP accumulation to a greater extent in the mTALH (Δ -51%) than in the oMCT (Δ -25%). The mechanism of attenuation differed between segments, and could not be attributed to either direct inhibition of adenylate cyclase activity nor direct potentiation of cAMP-phosphodiesterase activity. These data suggest that the gentamicin-induced decrease in renal concentrating ability may be due at least in part to reduced AVP-dependent cAMP accumulation in the oMCT and mTALH.",

keywords = "(thick ascending limb of), 5′-cyclic monophosphate, AVP, Adenosine 3′, Aminogiycosides, Collecting tubule, Henle's loop, [Arg]vasopressin",

author = "David Kidwell and Sriram Subramaniam and Cobern Ott and Brian Jackson",

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T1 - Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats

AU - Kidwell, David

AU - Subramaniam, Sriram

AU - Ott, Cobern

AU - Jackson, Brian

PY - 1990/12/4

Y1 - 1990/12/4

N2 - We have examined the effects of chronic gentamicin treatment on arginine8-vasopressin (AVP)-dependent cyclic AMP (cAMP) metabolism in rat medullary collecting tubules (oMCT) and medullary thick ascending limbs of Henle's loop (mTALH). Gentamicin attenuated AVP-stimulated cAMP accumulation to a greater extent in the mTALH (Δ -51%) than in the oMCT (Δ -25%). The mechanism of attenuation differed between segments, and could not be attributed to either direct inhibition of adenylate cyclase activity nor direct potentiation of cAMP-phosphodiesterase activity. These data suggest that the gentamicin-induced decrease in renal concentrating ability may be due at least in part to reduced AVP-dependent cAMP accumulation in the oMCT and mTALH.

AB - We have examined the effects of chronic gentamicin treatment on arginine8-vasopressin (AVP)-dependent cyclic AMP (cAMP) metabolism in rat medullary collecting tubules (oMCT) and medullary thick ascending limbs of Henle's loop (mTALH). Gentamicin attenuated AVP-stimulated cAMP accumulation to a greater extent in the mTALH (Δ -51%) than in the oMCT (Δ -25%). The mechanism of attenuation differed between segments, and could not be attributed to either direct inhibition of adenylate cyclase activity nor direct potentiation of cAMP-phosphodiesterase activity. These data suggest that the gentamicin-induced decrease in renal concentrating ability may be due at least in part to reduced AVP-dependent cAMP accumulation in the oMCT and mTALH.

KW - (thick ascending limb of)

KW - 5′-cyclic monophosphate

KW - AVP

KW - Adenosine 3′

KW - Aminogiycosides

KW - Collecting tubule

KW - Henle's loop

KW - [Arg]vasopressin

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JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 3

ER -

Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats

Abstract

Keywords

ASJC Scopus subject areas

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