Improved method for injecting fungal inoculum into corn ears

N. R. Anderson, J. D. Ravellette, K. A. Wise

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Corn (Zea mays L.) ear rot pathogens reduce yield and grain quality annually, and research on these pathogens and their interactions with the host can require inoculation of fungal material into the ears. A new system of mechanically inoculating corn ears was designed using a hydration backpack and auto-filling vaccinator with a needle attached. The efficiency of the new system was compared to a previously established inoculation method using a manual syringe method with an 18-gauge blunt end needle attached to a 60-ml syringe, with inoculum carried in a plastic beaker. Inoculation methods were tested by comparing the time to inoculate 100 corn ears with separate conidial suspensions of Fusarium verticillioides and F. graminearum in a replicated field trial. The new mechanical inoculation system reduced inoculation time by 42% (P = 0.0015) when compared to the manual syringe and needle method. Additional benefits of the new method include reduced risk of inoculum contamination, consistent inoculum volume per ear, and increased safety for personnel doing the inoculations.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalPlant Health Progress
Issue number3
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 The American Phytopathological Society.

ASJC Scopus subject areas

  • Plant Science
  • Horticulture


Dive into the research topics of 'Improved method for injecting fungal inoculum into corn ears'. Together they form a unique fingerprint.

Cite this