Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase

Marc T. Facciotti, Paul B. Bertain, Ling Yuan

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

The engineering of crops for selected fatty acid production is one of the major goals of plant biotechnology. The Garm FatA1, an acyl-acyl carrier protein (ACP) thioesterase isolated from Garcinia mangostana, generates an elevated stearate (18:0) phenotype in transgenic Brassica plants. By site- directed mutagenesis, we generated seven mutants that showed up to a 13-fold increase in specific enzyme activity toward 18:0-ACP in vitro. The seed- specific expression of mutant S111A/V193A in Brassica plants results in transgenic plants that accumulate 55-68% more stearate than plants expressing the wild-type enzyme. Our results demonstrate that a thioesterase can be engineered to increase specific activity and that its improved function demonstrated in vitro is retained in vivo.

Original languageEnglish
Pages (from-to)593-597
Number of pages5
JournalNature Biotechnology
Volume17
Issue number6
DOIs
StatePublished - Jun 1999

Keywords

  • Alanine scanning
  • Protein engineering
  • Site-directed mutagenesis
  • Stearate
  • Transgenic plants

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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