In situ spatial glycomic imaging of mouse and human Alzheimer's disease brains

Tara R. Hawkinson, Harrison A. Clarke, Lyndsay E.A. Young, Lindsey R. Conroy, Kia H. Markussen, Kayla M. Kerch, Lance Johnson, Peter T. Nelson, Chi Wang, Derek B. Allison, Matthew S. Gentry, Ramon Sun

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


N-linked protein glycosylation in the brain is an understudied facet of glucose utilization that impacts a myriad of cellular processes including resting membrane potential, axon firing, and synaptic vesicle trafficking. Currently, a spatial map of N-linked glycans within the normal and Alzheimer's disease (AD) human brain does not exist. A comprehensive analysis of the spatial N-linked glycome would improve our understanding of brain energy metabolism, linking metabolism to signaling events perturbed during AD progression, and could illuminate new therapeutic strategies. Herein we report an optimized in situ workflow for enzyme-assisted, matrix-assisted laser desorption and ionization (MALDI) mass spectrometry imaging (MSI) of brain N-linked glycans. Using this workflow, we spatially interrogated N-linked glycan heterogeneity in both mouse and human AD brains and their respective age-matched controls. We identified robust regional-specific N-linked glycan changes associated with AD in mice and humans. These data suggest that N-linked glycan dysregulation could be an underpinning of AD pathologies.

Original languageEnglish
Pages (from-to)1721-1735
Number of pages15
JournalAlzheimer's and Dementia
Issue number10
StatePublished - Oct 2022

Bibliographical note

Publisher Copyright:
© 2021 the Alzheimer's Association.


  • MALDI imaging
  • N-linked glycosylation
  • bioenergetics
  • carbohydrate metabolism
  • neuronal function
  • synaptic transmission

ASJC Scopus subject areas

  • Epidemiology
  • Health Policy
  • Developmental Neuroscience
  • Clinical Neurology
  • Geriatrics and Gerontology
  • Cellular and Molecular Neuroscience
  • Psychiatry and Mental health


Dive into the research topics of 'In situ spatial glycomic imaging of mouse and human Alzheimer's disease brains'. Together they form a unique fingerprint.

Cite this