In vitro analysis of Ah receptor domains involved in ligand-activated DNA recognition

K. M. Dolwick, H. I. Swanson, C. A. Bradfield

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin. In this report, we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this in vitro system, we were able to reconstitute agonist binding to the AHR and agonist-induced AHR-ARNT recognition of a cognate DNA enhancer sequence. Expression of AHR deletion mutants revealed the location of N-terminal domains responsible for ligand and DNA recognition and C-terminal domains that play roles in agonist-induced DNA recognition.

Original languageEnglish
Pages (from-to)8566-8570
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
StatePublished - Sep 15 1993

ASJC Scopus subject areas

  • General

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