In vitro assays to identify antibiotics targeting DNA metabolism

Allan H. Pang; Sylvie Garneau-Tsodikova; Oleg V. Tsodikov

doi:10.1007/978-1-4939-6634-9_11

In vitro assays to identify antibiotics targeting DNA metabolism

Allan H. Pang, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

DNA metabolism embodies a number of biochemical pathways, which include targets of clinically used antibiotics as well as those that are only being explored as potential targets for inhibitory compounds. We give an overview of representative cell-based and enzymatic assays suitable for high-throughput-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	175-200
Number of pages	26
DOIs	https://doi.org/10.1007/978-1-4939-6634-9_11
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1520
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2017.

Keywords

DNA recombination
DNA replication
High-throughput assay
Inhibitor discovery
Resistance

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-6634-9_11

Cite this

@inbook{335ce8f947244a4cbf4578e1f2c59395,

title = "In vitro assays to identify antibiotics targeting DNA metabolism",

abstract = "DNA metabolism embodies a number of biochemical pathways, which include targets of clinically used antibiotics as well as those that are only being explored as potential targets for inhibitory compounds. We give an overview of representative cell-based and enzymatic assays suitable for high-throughput-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.",

keywords = "DNA recombination, DNA replication, High-throughput assay, Inhibitor discovery, Resistance",

author = "Pang, {Allan H.} and Sylvie Garneau-Tsodikova and Tsodikov, {Oleg V.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

year = "2017",

doi = "10.1007/978-1-4939-6634-9_11",

language = "English",

series = "Methods in Molecular Biology",

pages = "175--200",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - In vitro assays to identify antibiotics targeting DNA metabolism

AU - Pang, Allan H.

AU - Garneau-Tsodikova, Sylvie

AU - Tsodikov, Oleg V.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2017.

PY - 2017

Y1 - 2017

N2 - DNA metabolism embodies a number of biochemical pathways, which include targets of clinically used antibiotics as well as those that are only being explored as potential targets for inhibitory compounds. We give an overview of representative cell-based and enzymatic assays suitable for high-throughput-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.

AB - DNA metabolism embodies a number of biochemical pathways, which include targets of clinically used antibiotics as well as those that are only being explored as potential targets for inhibitory compounds. We give an overview of representative cell-based and enzymatic assays suitable for high-throughput-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.

KW - DNA recombination

KW - DNA replication

KW - High-throughput assay

KW - Inhibitor discovery

KW - Resistance

UR - http://www.scopus.com/inward/record.url?scp=84996917481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84996917481&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6634-9_11

DO - 10.1007/978-1-4939-6634-9_11

M3 - Chapter

C2 - 27873253

AN - SCOPUS:84996917481

T3 - Methods in Molecular Biology

SP - 175

EP - 200

BT - Methods in Molecular Biology

ER -

In vitro assays to identify antibiotics targeting DNA metabolism

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this