In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products

Steffanie V. Burk; Sriveny Dangoudoubiyam; Tammy Brewster-Barnes; Uneeda K. Bryant; Daniel K. Howe; Craig N. Carter; Eric S. Vanzant; Robert J. Harmon; Kevin R. Kazacos; Mary G. Rossano

doi:10.1007/s00436-014-4097-0

In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products

Steffanie V. Burk, Sriveny Dangoudoubiyam, Tammy Brewster-Barnes, Uneeda K. Bryant, Daniel K. Howe, Craig N. Carter, Eric S. Vanzant, Robert J. Harmon, Kevin R. Kazacos, Mary G. Rossano

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Currently, diagnosis of Parascaris equorum infection in equids is limited to patent infections. The goals of this study were to culture P. equorum larvae in vitro and identify excretory-secretory (ES) products for prepatent diagnostic testing. Parascaris equorum L2/L3 larvae were hatched and cultured for up to 3 weeks for ES product collection. Fifth stage (L5) P. equorum were also cultured for ES product collection. Examination of ES fractions by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and silver stain revealed L2/L3 products ranging from 12–94 kDa and L5 products ranging from 12–189 kDa. Western blot analyses were conducted using polyclonal antibodies produced against P. equorum or Baylisascaris procyonis L2/L3 ES products, sera from rabbits inoculated with B. procyonis or Toxocara canis eggs, and sera from animals naturally infected with P. equorum or T. canis. Western blot results indicated parasite antigens migrating at 19 and 34 kDa may be useful for specifically detecting P. equorum infections.

Original language	English
Pages (from-to)	4217-4224
Number of pages	8
Journal	Parasitology Research
Volume	113
Issue number	11
DOIs	https://doi.org/10.1007/s00436-014-4097-0
State	Published - Oct 17 2014

Bibliographical note

Funding Information:
Acknowledgments We thank the University of Kentucky’s College of Agriculture for funding this research. The authors are grateful to Frank Berry, Bryan Cassill, Dr. Chris Christensen, Kristen Fox, Dr. Eugene Lyons, Dr. Martin Nielsen, Dr. William Silvia, Laura Strasinger, Dr. Elizabeth Ubelhor, and Stacy White for their assistance with specimen collection. We also thank Dr. Noel Inocencio, Jessica Gould, Lynn Ennis, and Sara Tanner for sharing their laboratory expertise.

Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.

Keywords

Antibody
Baylisascaris procyonis
Equine
Parascaris equorum
Toxocara canis
Western blot

ASJC Scopus subject areas

Parasitology
Veterinary (all)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-014-4097-0

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title = "In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products",

abstract = "Currently, diagnosis of Parascaris equorum infection in equids is limited to patent infections. The goals of this study were to culture P. equorum larvae in vitro and identify excretory-secretory (ES) products for prepatent diagnostic testing. Parascaris equorum L2/L3 larvae were hatched and cultured for up to 3 weeks for ES product collection. Fifth stage (L5) P. equorum were also cultured for ES product collection. Examination of ES fractions by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and silver stain revealed L2/L3 products ranging from 12–94 kDa and L5 products ranging from 12–189 kDa. Western blot analyses were conducted using polyclonal antibodies produced against P. equorum or Baylisascaris procyonis L2/L3 ES products, sera from rabbits inoculated with B. procyonis or Toxocara canis eggs, and sera from animals naturally infected with P. equorum or T. canis. Western blot results indicated parasite antigens migrating at 19 and 34 kDa may be useful for specifically detecting P. equorum infections.",

keywords = "Antibody, Baylisascaris procyonis, Equine, Parascaris equorum, Toxocara canis, Western blot",

author = "Burk, {Steffanie V.} and Sriveny Dangoudoubiyam and Tammy Brewster-Barnes and Bryant, {Uneeda K.} and Howe, {Daniel K.} and Carter, {Craig N.} and Vanzant, {Eric S.} and Harmon, {Robert J.} and Kazacos, {Kevin R.} and Rossano, {Mary G.}",

note = "Funding Information: Acknowledgments We thank the University of Kentucky{\textquoteright}s College of Agriculture for funding this research. The authors are grateful to Frank Berry, Bryan Cassill, Dr. Chris Christensen, Kristen Fox, Dr. Eugene Lyons, Dr. Martin Nielsen, Dr. William Silvia, Laura Strasinger, Dr. Elizabeth Ubelhor, and Stacy White for their assistance with specimen collection. We also thank Dr. Noel Inocencio, Jessica Gould, Lynn Ennis, and Sara Tanner for sharing their laboratory expertise. Publisher Copyright: {\textcopyright} 2014, Springer-Verlag Berlin Heidelberg.",

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doi = "10.1007/s00436-014-4097-0",

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AU - Brewster-Barnes, Tammy

AU - Bryant, Uneeda K.

AU - Howe, Daniel K.

AU - Carter, Craig N.

AU - Vanzant, Eric S.

AU - Harmon, Robert J.

AU - Kazacos, Kevin R.

AU - Rossano, Mary G.

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N2 - Currently, diagnosis of Parascaris equorum infection in equids is limited to patent infections. The goals of this study were to culture P. equorum larvae in vitro and identify excretory-secretory (ES) products for prepatent diagnostic testing. Parascaris equorum L2/L3 larvae were hatched and cultured for up to 3 weeks for ES product collection. Fifth stage (L5) P. equorum were also cultured for ES product collection. Examination of ES fractions by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and silver stain revealed L2/L3 products ranging from 12–94 kDa and L5 products ranging from 12–189 kDa. Western blot analyses were conducted using polyclonal antibodies produced against P. equorum or Baylisascaris procyonis L2/L3 ES products, sera from rabbits inoculated with B. procyonis or Toxocara canis eggs, and sera from animals naturally infected with P. equorum or T. canis. Western blot results indicated parasite antigens migrating at 19 and 34 kDa may be useful for specifically detecting P. equorum infections.

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In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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