Coculture of stallion sperm with monolayers of equine oviductal epithelia cells (OEC) was evaluated. Monolayers were obtained from frozen-thawed OEC. Live sperm attached to the OEC in vitro, whereas sperm killed by heat treatment or glutaraldehyde fixation did not. Sperm attached to OEC showed flagellar motion for 4dm vitro, during which time they gradually became released. Scanning electronmicrographs showed an intimate association between the sperm and OEC. Incubation of sperm for 4 h with either control, heparinized or OEC-conditioned medium (Tyrode's albumin lactate phosphate) resulted in more uncapacitated sperm, as determined by chlortetracycline staining patterns. The OEC-conditioned medium caused similar capacitation-like changes to those seen with heparin. Sperm viability as determined by Hoechst 33258 staining was not significantly affected by media type.
|Number of pages||8|
|Journal||Systems Biology in Reproductive Medicine|
|State||Published - 1993|
Bibliographical noteFunding Information:
Acknowledgement. This study was supported by NIH HD00884, the Harry M. Zweig Memorial Fund, and the American Quarter Horse Association. This investigation was presented in part at the MC Chang Festschrift, Tossa de Mar, Spain, August, 1992.
- Chlortetracycline stain
- In vitro fertilization
- Oviduct proteins
ASJC Scopus subject areas
- Reproductive Medicine