In vitro ligation of ureters and urethra modulates fetal mouse bladder explants development

Andy Beauboeuf, Sara Ordille, Deborah R. Erickson, H. Paul Ehrlich

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The compliance of the bladder which accommodates the holding and voiding of urine is influenced by the amount and type of collagen deposited as well as the packing and organization of collagen fiber bundles. During fetal development, the accumulation of urine within the bladder lumen is associated with the maturation of the bladder's wall. Fetal mouse bladders can undergo maturation as organ cultured explants in defined medium. Polarized light optics of Sirius red-stained sections of fetal mouse bladders in organ culture for 4 days showed that the ligation of both ureters and urethra promoted more orderly packing of collagen fiber bundles within the luminal edge of the lamina propria compared to unligated bladder explants. It is proposed that ligation causes differences in the development and organization of the collagen fiber bundles within the bladder wall. These differences are due to either increases in intravesical pressure, the accumulation of growth factors within the lumen or a combination of both.

Original languageEnglish
Pages (from-to)531-536
Number of pages6
JournalTissue and Cell
Issue number5
StatePublished - 1998

Bibliographical note

Funding Information:
This work was partially supported by NIH grants DK 51381 and GM 41343.


  • Bladder development
  • Collagen
  • Fetal mouse
  • Organ culture

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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