@article{6bd73e7ce1fc4a71a767dbb178f2018d,
title = "In vivo electrochemical measurements of serotonin clearance in rat striatum: Effects of neonatal 6-hydroxydopamine-induced serotonin hyperinnervation and serotonin uptake inhibitors",
abstract = "Diffusion and clearance of extracellular serotonin (5-HT) was examined using in vivo chronoamperometry with 'delayed-pulse' recordings after pressure ejections of 1 to 60 picomoles 5-HT into rat striatum at a fixed distance from a Nafion-coated carbon fiber electrode. Signals obtained were identified based on the signal characteristics to consist of 5-HT. Clearance times of 5-HT decreased, while amplitudes and rise times increased with serotonergic hyperinnervation induced by neonatal 6-hydroxydopamine (6-OHDA) lesions of dopamine (DA) neurons. Local applications of the 5-HT uptake inhibitors zimelidine or fluoxetine, in conjunction with 5-HT ejections, produced increased clearance times in both normal and 6-OHDA-treated animals. Thus, direct in vivo evidence was obtained for the importance of high affinity nerve terminal uptake as a key mechanism for clearance of 5-HT from the extracellular space. Inhibitors of 5-HT uptake appear to prolong the extracellular presence of 5-HT by increasing its clearance time.",
keywords = "Clearance, Diffusion, Hyperinnervation, In vivo electrochemistry, Serotonin, Uptake",
author = "J. Luthman and Friedemann, {M. N.} and Hoffer, {B. J.} and Gerhardt, {G. A.}",
note = "Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",
year = "1997",
doi = "10.1007/BF01277658",
language = "English",
volume = "104",
pages = "379--397",
number = "4-5",
}
