In vivo intra-luteal implants of prostaglandin (PG) E 1 or E 2 (PGE 1, PGE 2) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH

Yoshie S. Weems; J. Alejandro Arreguin-Arevalo; Torrance M. Nett; Rhonda C. Vann; Stephen P. Ford; Phillip J. Bridges; Thomas H. Welsh; Andrew W. Lewis; Don A. Neuendorff; Ronald D. Randel; Charles W. Weems

doi:10.1016/j.prostaglandins.2011.05.002

In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH

Yoshie S. Weems, J. Alejandro Arreguin-Arevalo, Torrance M. Nett, Rhonda C. Vann, Stephen P. Ford, Phillip J. Bridges, Thomas H. Welsh, Andrew W. Lewis, Don A. Neuendorff, Ronald D. Randel, Charles W. Weems

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Previously, it was reported that chronic intra-uterine infusion of PGE ₁ or PGE ₂ every four hours inhibited luteolysis in ewes. However, estradiol-17β or PGE ₂ given intra-uterine every 8 h did not inhibit luteolysis in heifers, but infusion of estradiol + PGE ₂ inhibited luteolysis in heifers. The objective of this experiment was to determine whether and how intra-luteal implants containing PGE ₁ or PGE ₂ prevent luteolysis in Angus or Brahman cows. On day-13 post-estrus, Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE ₁, or PGE ₂ and corpora lutea were retrieved on day-19. Coccygeal blood was collected daily for analysis for progesterone. Breed did not influence the effect of PGE ₁ or PGE ₂ on luteal mRNA for LH receptors or unoccupied or occupied luteal LH receptors did not differ (P > 0.05) so the data were pooled. Luteal weights of Vehicle-treated Angus or Brahman cows from days-13-19 were lower (P < 0.05) than those treated with intra-luteal implants containing PGE ₁ or PGE ₂. Day-13 Angus luteal weights were heavier (P < 0.05) than Vehicle-treated Angus cows on day-19 and luteal weights of day-13 corpora lutea were similar (P > 0.05) to Angus cows on day-19 treated with intra-luteal implants containing PGE ₁ or PGE ₂. Profiles of circulating progesterone in Angus or Brahman cows treated with intra-luteal implants containing PGE ₁ or PGE ₂ differed (P < 0.05) from controls, but profiles of progesterone did not differ (P > 0.05) between breeds or between cows treated with intra-luteal implants containing PGE ₁ or PGE ₂. Intra-luteal implants containing PGE ₁ or PGE ₂ prevented (P < 0.05) loss of luteal mRNA for LH receptors and unoccupied or occupied receptors for LH compared to controls. It is concluded that PGE ₁ or PGE ₂ alone delays luteolysis regardless of breed. We also conclude that either PGE ₁ or PGE ₂ prevented luteolysis in cows by up-regulating expression of mRNA for LH receptors and by preventing loss of unoccupied and occupied LH receptors in luteal tissue.

Original language	English
Pages (from-to)	35-44
Number of pages	10
Journal	Prostaglandins and Other Lipid Mediators
Volume	95
Issue number	1-4
DOIs	https://doi.org/10.1016/j.prostaglandins.2011.05.002
State	Published - Aug 2011

Bibliographical note

Funding Information:
The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. Antisera for progesterone were kindly provided by Dr. R.L. Butcher, West Virginia University, Morgantown, West Virginia. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems (CSREES 2006-04752) and USDA Regional Research Project W-112 to C.W. Weems (HAW-259) at the CollegeTropical Agriculture and Human Resources.

Keywords

Angus
Brahman
Corpus luteum
Cow
LH receptors
PGE
PGE
mRNA LH receptors

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

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Weems, Y. S., Arreguin-Arevalo, J. A., Nett, T. M., Vann, R. C., Ford, S. P., Bridges, P. J., Welsh, T. H., Lewis, A. W., Neuendorff, D. A., Randel, R. D., & Weems, C. W. (2011). In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH. Prostaglandins and Other Lipid Mediators, 95(1-4), 35-44. https://doi.org/10.1016/j.prostaglandins.2011.05.002

Weems, Yoshie S. ; Arreguin-Arevalo, J. Alejandro ; Nett, Torrance M. et al. / In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH. In: Prostaglandins and Other Lipid Mediators. 2011 ; Vol. 95, No. 1-4. pp. 35-44.

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title = "In vivo intra-luteal implants of prostaglandin (PG) E 1 or E 2 (PGE 1, PGE 2) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH",

abstract = "Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every four hours inhibited luteolysis in ewes. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in heifers, but infusion of estradiol + PGE 2 inhibited luteolysis in heifers. The objective of this experiment was to determine whether and how intra-luteal implants containing PGE 1 or PGE 2 prevent luteolysis in Angus or Brahman cows. On day-13 post-estrus, Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Coccygeal blood was collected daily for analysis for progesterone. Breed did not influence the effect of PGE 1 or PGE 2 on luteal mRNA for LH receptors or unoccupied or occupied luteal LH receptors did not differ (P > 0.05) so the data were pooled. Luteal weights of Vehicle-treated Angus or Brahman cows from days-13-19 were lower (P < 0.05) than those treated with intra-luteal implants containing PGE 1 or PGE 2. Day-13 Angus luteal weights were heavier (P < 0.05) than Vehicle-treated Angus cows on day-19 and luteal weights of day-13 corpora lutea were similar (P > 0.05) to Angus cows on day-19 treated with intra-luteal implants containing PGE 1 or PGE 2. Profiles of circulating progesterone in Angus or Brahman cows treated with intra-luteal implants containing PGE 1 or PGE 2 differed (P < 0.05) from controls, but profiles of progesterone did not differ (P > 0.05) between breeds or between cows treated with intra-luteal implants containing PGE 1 or PGE 2. Intra-luteal implants containing PGE 1 or PGE 2 prevented (P < 0.05) loss of luteal mRNA for LH receptors and unoccupied or occupied receptors for LH compared to controls. It is concluded that PGE 1 or PGE 2 alone delays luteolysis regardless of breed. We also conclude that either PGE 1 or PGE 2 prevented luteolysis in cows by up-regulating expression of mRNA for LH receptors and by preventing loss of unoccupied and occupied LH receptors in luteal tissue.",

keywords = "Angus, Brahman, Corpus luteum, Cow, LH receptors, PGE, PGE, mRNA LH receptors",

author = "Weems, {Yoshie S.} and Arreguin-Arevalo, {J. Alejandro} and Nett, {Torrance M.} and Vann, {Rhonda C.} and Ford, {Stephen P.} and Bridges, {Phillip J.} and Welsh, {Thomas H.} and Lewis, {Andrew W.} and Neuendorff, {Don A.} and Randel, {Ronald D.} and Weems, {Charles W.}",

note = "Funding Information: The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. Antisera for progesterone were kindly provided by Dr. R.L. Butcher, West Virginia University, Morgantown, West Virginia. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems (CSREES 2006-04752) and USDA Regional Research Project W-112 to C.W. Weems (HAW-259) at the CollegeTropical Agriculture and Human Resources. ",

year = "2011",

month = aug,

doi = "10.1016/j.prostaglandins.2011.05.002",

language = "English",

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pages = "35--44",

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Weems, YS, Arreguin-Arevalo, JA, Nett, TM, Vann, RC, Ford, SP, Bridges, PJ, Welsh, TH, Lewis, AW, Neuendorff, DA, Randel, RD & Weems, CW 2011, 'In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH', Prostaglandins and Other Lipid Mediators, vol. 95, no. 1-4, pp. 35-44. https://doi.org/10.1016/j.prostaglandins.2011.05.002

In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH. / Weems, Yoshie S.; Arreguin-Arevalo, J. Alejandro; Nett, Torrance M. et al.
In: Prostaglandins and Other Lipid Mediators, Vol. 95, No. 1-4, 08.2011, p. 35-44.

Research output: Contribution to journal › Article › peer-review

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AU - Weems, Yoshie S.

AU - Arreguin-Arevalo, J. Alejandro

AU - Nett, Torrance M.

AU - Vann, Rhonda C.

AU - Ford, Stephen P.

AU - Bridges, Phillip J.

AU - Welsh, Thomas H.

AU - Lewis, Andrew W.

AU - Neuendorff, Don A.

AU - Randel, Ronald D.

AU - Weems, Charles W.

N1 - Funding Information: The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. Antisera for progesterone were kindly provided by Dr. R.L. Butcher, West Virginia University, Morgantown, West Virginia. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems (CSREES 2006-04752) and USDA Regional Research Project W-112 to C.W. Weems (HAW-259) at the CollegeTropical Agriculture and Human Resources.

PY - 2011/8

Y1 - 2011/8

N2 - Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every four hours inhibited luteolysis in ewes. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in heifers, but infusion of estradiol + PGE 2 inhibited luteolysis in heifers. The objective of this experiment was to determine whether and how intra-luteal implants containing PGE 1 or PGE 2 prevent luteolysis in Angus or Brahman cows. On day-13 post-estrus, Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Coccygeal blood was collected daily for analysis for progesterone. Breed did not influence the effect of PGE 1 or PGE 2 on luteal mRNA for LH receptors or unoccupied or occupied luteal LH receptors did not differ (P > 0.05) so the data were pooled. Luteal weights of Vehicle-treated Angus or Brahman cows from days-13-19 were lower (P < 0.05) than those treated with intra-luteal implants containing PGE 1 or PGE 2. Day-13 Angus luteal weights were heavier (P < 0.05) than Vehicle-treated Angus cows on day-19 and luteal weights of day-13 corpora lutea were similar (P > 0.05) to Angus cows on day-19 treated with intra-luteal implants containing PGE 1 or PGE 2. Profiles of circulating progesterone in Angus or Brahman cows treated with intra-luteal implants containing PGE 1 or PGE 2 differed (P < 0.05) from controls, but profiles of progesterone did not differ (P > 0.05) between breeds or between cows treated with intra-luteal implants containing PGE 1 or PGE 2. Intra-luteal implants containing PGE 1 or PGE 2 prevented (P < 0.05) loss of luteal mRNA for LH receptors and unoccupied or occupied receptors for LH compared to controls. It is concluded that PGE 1 or PGE 2 alone delays luteolysis regardless of breed. We also conclude that either PGE 1 or PGE 2 prevented luteolysis in cows by up-regulating expression of mRNA for LH receptors and by preventing loss of unoccupied and occupied LH receptors in luteal tissue.

AB - Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every four hours inhibited luteolysis in ewes. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in heifers, but infusion of estradiol + PGE 2 inhibited luteolysis in heifers. The objective of this experiment was to determine whether and how intra-luteal implants containing PGE 1 or PGE 2 prevent luteolysis in Angus or Brahman cows. On day-13 post-estrus, Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Coccygeal blood was collected daily for analysis for progesterone. Breed did not influence the effect of PGE 1 or PGE 2 on luteal mRNA for LH receptors or unoccupied or occupied luteal LH receptors did not differ (P > 0.05) so the data were pooled. Luteal weights of Vehicle-treated Angus or Brahman cows from days-13-19 were lower (P < 0.05) than those treated with intra-luteal implants containing PGE 1 or PGE 2. Day-13 Angus luteal weights were heavier (P < 0.05) than Vehicle-treated Angus cows on day-19 and luteal weights of day-13 corpora lutea were similar (P > 0.05) to Angus cows on day-19 treated with intra-luteal implants containing PGE 1 or PGE 2. Profiles of circulating progesterone in Angus or Brahman cows treated with intra-luteal implants containing PGE 1 or PGE 2 differed (P < 0.05) from controls, but profiles of progesterone did not differ (P > 0.05) between breeds or between cows treated with intra-luteal implants containing PGE 1 or PGE 2. Intra-luteal implants containing PGE 1 or PGE 2 prevented (P < 0.05) loss of luteal mRNA for LH receptors and unoccupied or occupied receptors for LH compared to controls. It is concluded that PGE 1 or PGE 2 alone delays luteolysis regardless of breed. We also conclude that either PGE 1 or PGE 2 prevented luteolysis in cows by up-regulating expression of mRNA for LH receptors and by preventing loss of unoccupied and occupied LH receptors in luteal tissue.

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KW - mRNA LH receptors

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Weems YS, Arreguin-Arevalo JA, Nett TM, Vann RC, Ford SP, Bridges PJ et al. In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. I. Luteal weight, circulating progesterone, mRNA for luteal luteinizing hormone (LH) receptor, and occupied and unoccupied luteal receptors for LH. Prostaglandins and Other Lipid Mediators. 2011 Aug;95(1-4):35-44. doi: 10.1016/j.prostaglandins.2011.05.002