In vivo intra-luteal implants of prostaglandin (PG) E 1 or E 2 (PGE 1, PGE 2) prevent luteolysis in cows. II: MRNA for PGF 2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue

Yoshie S. Weems; Phillip J. Bridges; Myoungkun Jeoung; J. Alejandro Arreguin-Arevalo; Torrance M. Nett; Rhonda C. Vann; Stephen P. Ford; Andrew W. Lewis; Don A. Neuendorff; Thomas H. Welsh; Ronald D. Randel; Charles W. Weems

doi:10.1016/j.prostaglandins.2011.11.006

In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II: MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue

Yoshie S. Weems, Phillip J. Bridges, Myoungkun Jeoung, J. Alejandro Arreguin-Arevalo, Torrance M. Nett, Rhonda C. Vann, Stephen P. Ford, Andrew W. Lewis, Don A. Neuendorff, Thomas H. Welsh, Ronald D. Randel, Charles W. Weems

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Previously, it was reported that chronic intra-uterine infusion of PGE ₁ or PGE ₂ every 4 h inhibited luteolysis in ewes by altering luteal mRNA for luteinizing hormone (LH) receptors and unoccupied and occupied luteal LH receptors. However, estradiol-17β or PGE ₂ given intra-uterine every 8 h did not inhibit luteolysis in cows, but infusion of estradiol + PGE ₂ inhibited luteolysis. In contrast, intra-luteal implants containing PGE ₁ or PGE ₂ in Angus or Brahman cows also inhibited the decline in circulating progesterone, mRNA for LH receptors, and loss of unoccupied and occupied receptors for LH to prevent luteolysis. The objective of this experiment was to determine how intra-luteal implants of PGE ₁ or PGE ₂ alter mRNA for prostanoid receptors and how this could influence luteolysis in Brahman or Angus cows. On day-13 Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE ₁, or PGE ₂ and corpora lutea were retrieved on day-19. Corpora lutea slices were analyzed for mRNA for prostanoid receptors (FP, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4) by RT-PCR. Day-13 Angus cow luteal tissue served as pre-luteolytic controls. mRNA for FP receptors decreased in day-19 Vehicle controls compared to day-13 Vehicle controls regardless of breed. PGE ₁ and PGE ₂ up-regulated FP gene expression on day-19 compared to day-19 Vehicle controls regardless of breed. EP1 mRNA was not altered by any treatment. PGE ₁ and PGE ₂ down-regulated EP2 and EP4 mRNA compared to day-19 Vehicle controls regardless of breed. PGE ₁ or PGE ₂ up-regulated mRNA EP3B receptor subtype compared to day-19 Vehicle control cows regardless of breed. The similarities in relative gene expression profiles induced by PGE ₁ and PGE ₂ support their agonistic effects. We conclude that both PGE ₁ and PGE ₂ may prevent luteolysis by altering expression of mRNA for prostanoid receptors, which is correlated with changes in luteal mRNA for LH receptors reported previously in these same cows to prevent luteolysis.

Original language	English
Pages (from-to)	60-65
Number of pages	6
Journal	Prostaglandins and Other Lipid Mediators
Volume	97
Issue number	1-2
DOIs	https://doi.org/10.1016/j.prostaglandins.2011.11.006
State	Published - Jan 2012

Bibliographical note

Funding Information:
The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems ( CSREES 2006-04752 ) and USDA Regional Research Project W-112 to C.W. Weems ( HAW-259 ) at the College of Tropical Agriculture and Human Resources.

Keywords

Angus
Brahman
Corpus luteum
Cow
PGE
PGE
mRNA prostanoid receptors

ASJC Scopus subject areas

Biochemistry
Physiology
Pharmacology
Cell Biology

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Weems, Y. S., Bridges, P. J., Jeoung, M., Arreguin-Arevalo, J. A., Nett, T. M., Vann, R. C., Ford, S. P., Lewis, A. W., Neuendorff, D. A., Welsh, T. H., Randel, R. D., & Weems, C. W. (2012). In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II: MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue. Prostaglandins and Other Lipid Mediators, 97(1-2), 60-65. https://doi.org/10.1016/j.prostaglandins.2011.11.006

Weems, Yoshie S. ; Bridges, Phillip J. ; Jeoung, Myoungkun et al. / In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II : MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue. In: Prostaglandins and Other Lipid Mediators. 2012 ; Vol. 97, No. 1-2. pp. 60-65.

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title = "In vivo intra-luteal implants of prostaglandin (PG) E 1 or E 2 (PGE 1, PGE 2) prevent luteolysis in cows. II: MRNA for PGF 2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue",

abstract = "Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every 4 h inhibited luteolysis in ewes by altering luteal mRNA for luteinizing hormone (LH) receptors and unoccupied and occupied luteal LH receptors. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in cows, but infusion of estradiol + PGE 2 inhibited luteolysis. In contrast, intra-luteal implants containing PGE 1 or PGE 2 in Angus or Brahman cows also inhibited the decline in circulating progesterone, mRNA for LH receptors, and loss of unoccupied and occupied receptors for LH to prevent luteolysis. The objective of this experiment was to determine how intra-luteal implants of PGE 1 or PGE 2 alter mRNA for prostanoid receptors and how this could influence luteolysis in Brahman or Angus cows. On day-13 Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Corpora lutea slices were analyzed for mRNA for prostanoid receptors (FP, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4) by RT-PCR. Day-13 Angus cow luteal tissue served as pre-luteolytic controls. mRNA for FP receptors decreased in day-19 Vehicle controls compared to day-13 Vehicle controls regardless of breed. PGE 1 and PGE 2 up-regulated FP gene expression on day-19 compared to day-19 Vehicle controls regardless of breed. EP1 mRNA was not altered by any treatment. PGE 1 and PGE 2 down-regulated EP2 and EP4 mRNA compared to day-19 Vehicle controls regardless of breed. PGE 1 or PGE 2 up-regulated mRNA EP3B receptor subtype compared to day-19 Vehicle control cows regardless of breed. The similarities in relative gene expression profiles induced by PGE 1 and PGE 2 support their agonistic effects. We conclude that both PGE 1 and PGE 2 may prevent luteolysis by altering expression of mRNA for prostanoid receptors, which is correlated with changes in luteal mRNA for LH receptors reported previously in these same cows to prevent luteolysis.",

keywords = "Angus, Brahman, Corpus luteum, Cow, PGE, PGE, mRNA prostanoid receptors",

author = "Weems, {Yoshie S.} and Bridges, {Phillip J.} and Myoungkun Jeoung and Arreguin-Arevalo, {J. Alejandro} and Nett, {Torrance M.} and Vann, {Rhonda C.} and Ford, {Stephen P.} and Lewis, {Andrew W.} and Neuendorff, {Don A.} and Welsh, {Thomas H.} and Randel, {Ronald D.} and Weems, {Charles W.}",

note = "Funding Information: The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems ( CSREES 2006-04752 ) and USDA Regional Research Project W-112 to C.W. Weems ( HAW-259 ) at the College of Tropical Agriculture and Human Resources.",

year = "2012",

month = jan,

doi = "10.1016/j.prostaglandins.2011.11.006",

language = "English",

volume = "97",

pages = "60--65",

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}

Weems, YS, Bridges, PJ, Jeoung, M, Arreguin-Arevalo, JA, Nett, TM, Vann, RC, Ford, SP, Lewis, AW, Neuendorff, DA, Welsh, TH, Randel, RD & Weems, CW 2012, 'In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II: MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue', Prostaglandins and Other Lipid Mediators, vol. 97, no. 1-2, pp. 60-65. https://doi.org/10.1016/j.prostaglandins.2011.11.006

In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II: MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue. / Weems, Yoshie S.; Bridges, Phillip J.; Jeoung, Myoungkun et al.
In: Prostaglandins and Other Lipid Mediators, Vol. 97, No. 1-2, 01.2012, p. 60-65.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - In vivo intra-luteal implants of prostaglandin (PG) E 1 or E 2 (PGE 1, PGE 2) prevent luteolysis in cows. II

T2 - MRNA for PGF 2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue

AU - Weems, Yoshie S.

AU - Bridges, Phillip J.

AU - Jeoung, Myoungkun

AU - Arreguin-Arevalo, J. Alejandro

AU - Nett, Torrance M.

AU - Vann, Rhonda C.

AU - Ford, Stephen P.

AU - Lewis, Andrew W.

AU - Neuendorff, Don A.

AU - Welsh, Thomas H.

AU - Randel, Ronald D.

AU - Weems, Charles W.

N1 - Funding Information: The authors would like to thank Mr. Wayne Toma (University of Hawaii) for assistance with statistical analysis and graphics. This paper is dedicated to the memory of Dr. Hal R. Behrman who has been a long time collaborator with Dr. Weems. This work was supported by USDA-CSREES Special Grants Program (TSTAR) administrated by the Pacific Basin Advisory Group (PBAG) grant to C.W. Weems ( CSREES 2006-04752 ) and USDA Regional Research Project W-112 to C.W. Weems ( HAW-259 ) at the College of Tropical Agriculture and Human Resources.

PY - 2012/1

Y1 - 2012/1

N2 - Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every 4 h inhibited luteolysis in ewes by altering luteal mRNA for luteinizing hormone (LH) receptors and unoccupied and occupied luteal LH receptors. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in cows, but infusion of estradiol + PGE 2 inhibited luteolysis. In contrast, intra-luteal implants containing PGE 1 or PGE 2 in Angus or Brahman cows also inhibited the decline in circulating progesterone, mRNA for LH receptors, and loss of unoccupied and occupied receptors for LH to prevent luteolysis. The objective of this experiment was to determine how intra-luteal implants of PGE 1 or PGE 2 alter mRNA for prostanoid receptors and how this could influence luteolysis in Brahman or Angus cows. On day-13 Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Corpora lutea slices were analyzed for mRNA for prostanoid receptors (FP, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4) by RT-PCR. Day-13 Angus cow luteal tissue served as pre-luteolytic controls. mRNA for FP receptors decreased in day-19 Vehicle controls compared to day-13 Vehicle controls regardless of breed. PGE 1 and PGE 2 up-regulated FP gene expression on day-19 compared to day-19 Vehicle controls regardless of breed. EP1 mRNA was not altered by any treatment. PGE 1 and PGE 2 down-regulated EP2 and EP4 mRNA compared to day-19 Vehicle controls regardless of breed. PGE 1 or PGE 2 up-regulated mRNA EP3B receptor subtype compared to day-19 Vehicle control cows regardless of breed. The similarities in relative gene expression profiles induced by PGE 1 and PGE 2 support their agonistic effects. We conclude that both PGE 1 and PGE 2 may prevent luteolysis by altering expression of mRNA for prostanoid receptors, which is correlated with changes in luteal mRNA for LH receptors reported previously in these same cows to prevent luteolysis.

AB - Previously, it was reported that chronic intra-uterine infusion of PGE 1 or PGE 2 every 4 h inhibited luteolysis in ewes by altering luteal mRNA for luteinizing hormone (LH) receptors and unoccupied and occupied luteal LH receptors. However, estradiol-17β or PGE 2 given intra-uterine every 8 h did not inhibit luteolysis in cows, but infusion of estradiol + PGE 2 inhibited luteolysis. In contrast, intra-luteal implants containing PGE 1 or PGE 2 in Angus or Brahman cows also inhibited the decline in circulating progesterone, mRNA for LH receptors, and loss of unoccupied and occupied receptors for LH to prevent luteolysis. The objective of this experiment was to determine how intra-luteal implants of PGE 1 or PGE 2 alter mRNA for prostanoid receptors and how this could influence luteolysis in Brahman or Angus cows. On day-13 Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE 1, or PGE 2 and corpora lutea were retrieved on day-19. Corpora lutea slices were analyzed for mRNA for prostanoid receptors (FP, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4) by RT-PCR. Day-13 Angus cow luteal tissue served as pre-luteolytic controls. mRNA for FP receptors decreased in day-19 Vehicle controls compared to day-13 Vehicle controls regardless of breed. PGE 1 and PGE 2 up-regulated FP gene expression on day-19 compared to day-19 Vehicle controls regardless of breed. EP1 mRNA was not altered by any treatment. PGE 1 and PGE 2 down-regulated EP2 and EP4 mRNA compared to day-19 Vehicle controls regardless of breed. PGE 1 or PGE 2 up-regulated mRNA EP3B receptor subtype compared to day-19 Vehicle control cows regardless of breed. The similarities in relative gene expression profiles induced by PGE 1 and PGE 2 support their agonistic effects. We conclude that both PGE 1 and PGE 2 may prevent luteolysis by altering expression of mRNA for prostanoid receptors, which is correlated with changes in luteal mRNA for LH receptors reported previously in these same cows to prevent luteolysis.

KW - Angus

KW - Brahman

KW - Corpus luteum

KW - Cow

KW - PGE

KW - mRNA prostanoid receptors

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Weems YS, Bridges PJ, Jeoung M, Arreguin-Arevalo JA, Nett TM, Vann RC et al. In vivo intra-luteal implants of prostaglandin (PG) E ₁ or E ₂ (PGE ₁, PGE ₂) prevent luteolysis in cows. II: MRNA for PGF _2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue. Prostaglandins and Other Lipid Mediators. 2012 Jan;97(1-2):60-65. doi: 10.1016/j.prostaglandins.2011.11.006