TY - JOUR
T1 - In vivo replication-deficient adenovirus vector-mediated transduction of the cytosine deaminase gene sensitizes glioma cells to 5-fluorocytosine
AU - Dong, Yonghe
AU - Wen, Patrick
AU - Manome, Yoshinobu
AU - Parr, Michael
AU - Hirshowitz, Ari
AU - Chen, Ling
AU - Hirschowitz, Edward A.
AU - Crystal, Ronald
AU - Weichselbaum, Ralph
AU - Kufe, Donald W.
AU - Fine, Howard A.
PY - 1996/4/10
Y1 - 1996/4/10
N2 - Viral vector-mediated transfer of chemosensitization genes represents a promising new approach to the treatment of cancer. Previous reports have demonstrated that transfection of the bacterial cytosine deaminase (cd) gene into mammalian cells can sensitize them to the otherwise nontoxic nucleoside, 5-fluorocytosine (5-FC). We now report that a replication-deficient adenovirus vector that transduces the cd gene (Ad.CMV-cd) highly sensitizes 9L gliosarcoma cells to 5-FC, and that gene transduction is associated with a potent bystander effect that is not dependent on direct cell-to-cell contact. Stereotactic injection of Ad.CMV-cd into established rat gliomas, followed by systemic administration of 5-FC in vivo, results in prolongation of survival.
AB - Viral vector-mediated transfer of chemosensitization genes represents a promising new approach to the treatment of cancer. Previous reports have demonstrated that transfection of the bacterial cytosine deaminase (cd) gene into mammalian cells can sensitize them to the otherwise nontoxic nucleoside, 5-fluorocytosine (5-FC). We now report that a replication-deficient adenovirus vector that transduces the cd gene (Ad.CMV-cd) highly sensitizes 9L gliosarcoma cells to 5-FC, and that gene transduction is associated with a potent bystander effect that is not dependent on direct cell-to-cell contact. Stereotactic injection of Ad.CMV-cd into established rat gliomas, followed by systemic administration of 5-FC in vivo, results in prolongation of survival.
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U2 - 10.1089/hum.1996.7.6-713
DO - 10.1089/hum.1996.7.6-713
M3 - Article
C2 - 8919593
AN - SCOPUS:0029995247
SN - 1043-0342
VL - 7
SP - 713
EP - 720
JO - Human Gene Therapy
JF - Human Gene Therapy
IS - 6
ER -
