In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells

Lei Qi, Teresa Knifley, Dava W. Piecoro, Piotr Rychahou, Jianrong Wu, Kathleen L. O’Connor, Min Chen

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based xenograft. Our protocol ensures sufficient establishment of the mouse xenografts and allows us to monitor tumor growth and spontaneous metastasis. This protocol could be adapted to other types of established cancer cell lines or primary cancer cells to study the mechanism of metastatic process as well as to test the effect of the potential anti-cancer agents on tumor growth and metastatic capacity.

Original languageEnglish
Article numbere3579
JournalBio-protocol
Volume10
Issue number7
DOIs
StatePublished - Apr 5 2020

Bibliographical note

Publisher Copyright:
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Keywords

  • Cell line xenograft
  • Implantation
  • Metastasis rate
  • Mouse
  • Patient-derived xenograft
  • Tumor growth

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Plant Science

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