In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells

Lei Qi; Teresa Knifley; Dava W. Piecoro; Piotr Rychahou; Jianrong Wu; Kathleen L. O’Connor; Min Chen

doi:10.21769/BioProtoc.3579

In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells

Lei Qi, Teresa Knifley, Dava W. Piecoro, Piotr Rychahou, Jianrong Wu, Kathleen L. O’Connor, Min Chen

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based xenograft. Our protocol ensures sufficient establishment of the mouse xenografts and allows us to monitor tumor growth and spontaneous metastasis. This protocol could be adapted to other types of established cancer cell lines or primary cancer cells to study the mechanism of metastatic process as well as to test the effect of the potential anti-cancer agents on tumor growth and metastatic capacity.

Original language	English
Article number	e3579
Journal	Bio-protocol
Volume	10
Issue number	7
DOIs	https://doi.org/10.21769/BioProtoc.3579
State	Published - Apr 5 2020

Bibliographical note

Publisher Copyright:
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Funding

This study was supported by American Cancer Society Institutional Research Grant IRG-85-001-25 (MC); the Kentucky Lung Cancer Research program (MC); the National Center for Advancing Translational Sciences National Institutes of Health UL1TR000117 (MC); the University of Kentucky Center for Cancer and Metabolism [P20GM121327] for providing services through the Imaging Core and for the pilot funding [no grant number, to MC]); and the National Institutes of Health R01 CA109136 (KLO). The Markey Cancer Center Biospecimen Procurement and Translational Pathology, the Biostatistics and Bioinformatics and Cancer Research Informatics Shared Resource Facilities are supported by National Institutes of Health [P30 CA177558]. This protocol was briefly described and originally published in Liu et al., 2019.

Funders	Funder number
Kentucky Lung Cancer Research Program
National Institutes of Health National Center for Advancing Translational Sciences	UL1TR000117
NIH-funded University of Kentucky Center for Cancer and Metabolism	P20GM121327
National Institutes of Health (NIH)	P30 CA177558, R01 CA109136
American Cancer Society	IRG-85-001-25

Keywords

Cell line xenograft
Implantation
Metastasis rate
Mouse
Patient-derived xenograft
Tumor growth

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
Plant Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21769/BioProtoc.3579

Cite this

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title = "In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells",

abstract = "Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based xenograft. Our protocol ensures sufficient establishment of the mouse xenografts and allows us to monitor tumor growth and spontaneous metastasis. This protocol could be adapted to other types of established cancer cell lines or primary cancer cells to study the mechanism of metastatic process as well as to test the effect of the potential anti-cancer agents on tumor growth and metastatic capacity.",

keywords = "Cell line xenograft, Implantation, Metastasis rate, Mouse, Patient-derived xenograft, Tumor growth",

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doi = "10.21769/BioProtoc.3579",

language = "English",

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T1 - In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells

AU - Qi, Lei

AU - Knifley, Teresa

AU - Piecoro, Dava W.

AU - Rychahou, Piotr

AU - Wu, Jianrong

AU - O’Connor, Kathleen L.

AU - Chen, Min

PY - 2020/4/5

Y1 - 2020/4/5

N2 - Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based xenograft. Our protocol ensures sufficient establishment of the mouse xenografts and allows us to monitor tumor growth and spontaneous metastasis. This protocol could be adapted to other types of established cancer cell lines or primary cancer cells to study the mechanism of metastatic process as well as to test the effect of the potential anti-cancer agents on tumor growth and metastatic capacity.

AB - Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based xenograft. Our protocol ensures sufficient establishment of the mouse xenografts and allows us to monitor tumor growth and spontaneous metastasis. This protocol could be adapted to other types of established cancer cell lines or primary cancer cells to study the mechanism of metastatic process as well as to test the effect of the potential anti-cancer agents on tumor growth and metastatic capacity.

KW - Cell line xenograft

KW - Implantation

KW - Metastasis rate

KW - Mouse

KW - Patient-derived xenograft

KW - Tumor growth

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In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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