Incomplete editing of rps12 transcripts results in the synthesis of polymorphic polypeptides in plant mitochondria

Christina G. Phreaner, Mark A. Williams, R. Michael Mulligan

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

C-to-U editing causes specific nucleotide changes in plant mitochondrial mRNAs that are required for the restoration of the evolutionary conserved amino acid sequence. Transcripts for the ribosomal protein S12 gene (rps12) have six C-to-U editing sites and are highly heterogeneous as a result of incomplete editing. Immunological analysis demonstrated that unedited or partially edited transcripts as well as edited mRNAs are translated. The edited rps12 translation product accumulate as ribosomal subunits, but the unedited rps12 translation products are present as unassembled subunits and are not detected in the ribosomes. Thus, gene expression is polymorphic as a result of incomplete C-to-U editing, and aberrant polypeptides are present from the translation of these mRNAs. However, because only the edited translation products accumulate in mitochondrial ribosomes, the overall expression of rps12 is rendered coherent by the selection of edited translation products for ribosomal biogenesis.

Original languageEnglish
Pages (from-to)107-117
Number of pages11
JournalPlant Cell
Volume8
Issue number1
DOIs
StatePublished - Jan 1996

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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