Increase in manganese superoxide dismutase activity in the mouse heart after X-irradiation

Larry W. Oberley; Daret Kasemset St Clair; Anne P. Autor; Terry D. Oberley

doi:10.1016/0003-9861(87)90082-8

Increase in manganese superoxide dismutase activity in the mouse heart after X-irradiation

Larry W. Oberley, Daret Kasemset St Clair, Anne P. Autor, Terry D. Oberley

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

Local X-irradiation of mouse heart caused a large increase in manganese superoxide dismutase activity (MnSOD) in this organ but not in copper and zinc containing superoxide dismutase (CuZn SOD) activity. MnSOD induction was both dose and time dependent. Another mitochondrial enzyme, citrate synthase, was not induced by X-irradiation. The amount of immunoreactive MnSOD also increased after X-irradiation, showing that the amount of MnSOD protein increased after X-irradiation. The response to X-irradiation was found to be biphasic-with one large peak and one smaller peak of manganese superoxide dismutase activity. The effect of various inhibitors of cellular activities on these two peaks of MnSOD activity was examined. Cycloheximide, a cytosolic protein synthesis inhibitor, abolished both peaks of MnSOD activity, while chloramphenicol, a mitochondrial protein synthesis inhibitor, has no effect on either peak. Actinomycin D, a RNA-synthesis inhibitor, lowered both peaks, but had more of an effect on the second peak than on the first. In vivo protein synthesis studies using [³H]arginine showed that an increase in new protein synthesis occurred during the time period of the second peak, but did not occur during the first peak. These results are consistent with the hypothesis that MnSOD induction occurs in two peaks with the first peak due to a preformed MnSOD protein or mRNA for MnSOD and the second peak due to an increase in new protein synthesis.

Original language	English
Pages (from-to)	69-80
Number of pages	12
Journal	Archives of Biochemistry and Biophysics
Volume	254
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(87)90082-8
State	Published - Apr 1987

Bibliographical note

Funding Information:
i This work was supported by a seed grant from the Division of Radiation Therapy, University of Iowa, and NIH Grant lROl-CA41267. ‘To whom correspondence should be addressed at 14 Medical Laboratories, Radiation Research Laboratory, University of Iowa, Iowa City, IA 52242. ’ Present address: Department of Biochemistry, Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, MA 02115. ’ Present address: Pulmonary Research Laboratory, St. Paul’s Hospital, University of British Columbia, Vancouver, British Columbia, Canada.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(87)90082-8

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@article{c2d0c6646a5c4ff689392d13ef8d282e,

title = "Increase in manganese superoxide dismutase activity in the mouse heart after X-irradiation",

abstract = "Local X-irradiation of mouse heart caused a large increase in manganese superoxide dismutase activity (MnSOD) in this organ but not in copper and zinc containing superoxide dismutase (CuZn SOD) activity. MnSOD induction was both dose and time dependent. Another mitochondrial enzyme, citrate synthase, was not induced by X-irradiation. The amount of immunoreactive MnSOD also increased after X-irradiation, showing that the amount of MnSOD protein increased after X-irradiation. The response to X-irradiation was found to be biphasic-with one large peak and one smaller peak of manganese superoxide dismutase activity. The effect of various inhibitors of cellular activities on these two peaks of MnSOD activity was examined. Cycloheximide, a cytosolic protein synthesis inhibitor, abolished both peaks of MnSOD activity, while chloramphenicol, a mitochondrial protein synthesis inhibitor, has no effect on either peak. Actinomycin D, a RNA-synthesis inhibitor, lowered both peaks, but had more of an effect on the second peak than on the first. In vivo protein synthesis studies using [3H]arginine showed that an increase in new protein synthesis occurred during the time period of the second peak, but did not occur during the first peak. These results are consistent with the hypothesis that MnSOD induction occurs in two peaks with the first peak due to a preformed MnSOD protein or mRNA for MnSOD and the second peak due to an increase in new protein synthesis.",

author = "Oberley, {Larry W.} and Clair, {Daret Kasemset St} and Autor, {Anne P.} and Oberley, {Terry D.}",

note = "Funding Information: i This work was supported by a seed grant from the Division of Radiation Therapy, University of Iowa, and NIH Grant lROl-CA41267. {\textquoteleft}To whom correspondence should be addressed at 14 Medical Laboratories, Radiation Research Laboratory, University of Iowa, Iowa City, IA 52242. {\textquoteright} Present address: Department of Biochemistry, Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, MA 02115. {\textquoteright} Present address: Pulmonary Research Laboratory, St. Paul{\textquoteright}s Hospital, University of British Columbia, Vancouver, British Columbia, Canada.",

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AU - Oberley, Terry D.

N1 - Funding Information: i This work was supported by a seed grant from the Division of Radiation Therapy, University of Iowa, and NIH Grant lROl-CA41267. ‘To whom correspondence should be addressed at 14 Medical Laboratories, Radiation Research Laboratory, University of Iowa, Iowa City, IA 52242. ’ Present address: Department of Biochemistry, Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, MA 02115. ’ Present address: Pulmonary Research Laboratory, St. Paul’s Hospital, University of British Columbia, Vancouver, British Columbia, Canada.

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N2 - Local X-irradiation of mouse heart caused a large increase in manganese superoxide dismutase activity (MnSOD) in this organ but not in copper and zinc containing superoxide dismutase (CuZn SOD) activity. MnSOD induction was both dose and time dependent. Another mitochondrial enzyme, citrate synthase, was not induced by X-irradiation. The amount of immunoreactive MnSOD also increased after X-irradiation, showing that the amount of MnSOD protein increased after X-irradiation. The response to X-irradiation was found to be biphasic-with one large peak and one smaller peak of manganese superoxide dismutase activity. The effect of various inhibitors of cellular activities on these two peaks of MnSOD activity was examined. Cycloheximide, a cytosolic protein synthesis inhibitor, abolished both peaks of MnSOD activity, while chloramphenicol, a mitochondrial protein synthesis inhibitor, has no effect on either peak. Actinomycin D, a RNA-synthesis inhibitor, lowered both peaks, but had more of an effect on the second peak than on the first. In vivo protein synthesis studies using [3H]arginine showed that an increase in new protein synthesis occurred during the time period of the second peak, but did not occur during the first peak. These results are consistent with the hypothesis that MnSOD induction occurs in two peaks with the first peak due to a preformed MnSOD protein or mRNA for MnSOD and the second peak due to an increase in new protein synthesis.

AB - Local X-irradiation of mouse heart caused a large increase in manganese superoxide dismutase activity (MnSOD) in this organ but not in copper and zinc containing superoxide dismutase (CuZn SOD) activity. MnSOD induction was both dose and time dependent. Another mitochondrial enzyme, citrate synthase, was not induced by X-irradiation. The amount of immunoreactive MnSOD also increased after X-irradiation, showing that the amount of MnSOD protein increased after X-irradiation. The response to X-irradiation was found to be biphasic-with one large peak and one smaller peak of manganese superoxide dismutase activity. The effect of various inhibitors of cellular activities on these two peaks of MnSOD activity was examined. Cycloheximide, a cytosolic protein synthesis inhibitor, abolished both peaks of MnSOD activity, while chloramphenicol, a mitochondrial protein synthesis inhibitor, has no effect on either peak. Actinomycin D, a RNA-synthesis inhibitor, lowered both peaks, but had more of an effect on the second peak than on the first. In vivo protein synthesis studies using [3H]arginine showed that an increase in new protein synthesis occurred during the time period of the second peak, but did not occur during the first peak. These results are consistent with the hypothesis that MnSOD induction occurs in two peaks with the first peak due to a preformed MnSOD protein or mRNA for MnSOD and the second peak due to an increase in new protein synthesis.

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Increase in manganese superoxide dismutase activity in the mouse heart after X-irradiation

Abstract

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