Increased Gene Expression of C1orf74 Is Associated with Poor Prognosis in Cervical Cancer

Preetiparna Parida, Shirley Lewis, Krishna Sharan, Mehta Vedant Kamal, Naveena A.N. Kumar, Vishwapriya M. Godkhindi, Sooryanarayana Varambally, Vivek M. Rangnekar, Mahadev Rao, Rama Rao Damerla

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

C1orf74, also known as URCL4, has been reported to have higher expression and be associated with poor prognosis in lung adenocarcinoma patients, and its role in regulation of the EGFR/AKT/mTORC1 pathway has been recently elucidated. In the current study, we used publicly available data and experimental validation of C1orf74 gene expression and its association with prognosis in cervical cancer patients. qRT-PCR was performed using RNA from cervical cancer cell lines and twenty-five cervical cancer patients. Data from TNMplot revealed that mRNA expression of the C1orf74 gene in primary tumor tissues, as well as metastatic tissues from cervical cancer patients, was significantly higher compared to normal cervical tissues. HPV-positive tumors had higher expression of this gene compared to HPV-negative tumors. qPCR analysis also demonstrated higher expression of C1orf74 in HPV-positive cervical cancer cell lines and most cervical cancer patients. The promoter methylation levels of the C1orf74 gene in cervical cancer tissues were lower compared to normal cervical tissues (p < 0.05). Collectively, our study indicates that higher expression of the C1orf74 gene caused by hypomethylation of its promoter is associated with poor overall survival in cervical cancer patients. Thus, C1orf74 is a novel prognostic marker in cervical cancer.

Original languageEnglish
Article number2530
JournalCells
Volume12
Issue number21
DOIs
StatePublished - Nov 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Keywords

  • C1orf74
  • cervical cancer
  • gene expression
  • prognosis
  • survival

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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