Increasing confidence of protein-protein interactomes.

Jin Chen, Hon Nian Chua, Wynne Hsu, Mong Li Lee, See Kiong Ng, Rintaro Saito, Wing Kin Sung, Limsoon Wong

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


High-throughput experimental methods, such as yeast-two-hybrid and phage display, have fairly high levels of false positives (and false negatives). Thus the list of protein-protein interactions detected by such experiments would need additional wet laboratory validation. It would be useful if the list could be prioritized in some way. Advances in computational techniques for assessing the reliability of protein-protein interactions detected by such high-throughput methods are reviewed in this paper, with a focus on techniques that rely only on topological information of the protein interaction network derived from such high-throughput experiments. In particular, we discuss indices that are abstract mathematical characterizations of networks of reliable protein-protein interactions--e.g., "interaction generality" (IG), "interaction reliability by alternative pathways" (IRAP), and "functional similarity weighting" (FSWeight). We also present indices that are based on explicit motifs associated with true-positive protein interactions--e.g., "new interaction generality" (IG2) and "meso-scale motifs" (NeMoFinder).

Original languageEnglish
Pages (from-to)284-297
Number of pages14
JournalGenome informatics. International Conference on Genome Informatics
Issue number2
StatePublished - 2006

ASJC Scopus subject areas

  • General Medicine


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