Inducible nitric oxide synthase in innate immune cells is important for restricting cyst formation of Toxoplasma gondii in the brain but not required for the protective immune process to remove the cysts

Qila Sa, Ashish Tiwari, Eri Ochiai, Jeremi Mullins, Yasuhiro Suzuki

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Significantly larger numbers of Toxoplasma gondii cysts were detected in the brains of RAG1 −/− NOS2 −/− than RAG1 −/− mice following infection. In contrast, the cyst numbers markedly decreased in a same manner in both strains of mice after receiving CD8 + immune T cells. Thus, NOS2-mediated innate immunity is important for inhibiting formation of cysts in the brain but not required for the T cell-initiated cyst removal, which is associated with phagocyte accumulation. Treatment with chloroquine, an inhibitor of endolysosomal acidification, partially but significantly inhibited the T cell-mediated cyst removal, suggesting that phagosome–lysosome fusion could be involved in the T. gondii cyst elimination.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalMicrobes and Infection
Volume20
Issue number4
DOIs
StatePublished - Apr 2018

Bibliographical note

Publisher Copyright:
© 2017 Institut Pasteur

Keywords

  • CD8 T cells
  • Cyst
  • Inducible nitric oxide synthase
  • Phagolysosome
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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