Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA

G. S. Gilkeson, J. P. Grudier, D. G. Karounos, D. S. Pisetsky

Research output: Contribution to journalArticlepeer-review

181 Scopus citations

Abstract

Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.

Original languageEnglish
Pages (from-to)1482-1486
Number of pages5
JournalJournal of Immunology
Volume142
Issue number5
StatePublished - 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA'. Together they form a unique fingerprint.

Cite this