Induction of choline acetyltransferase in the neuroblastoma x glioma cell line NG108-15

Louis B. Hersh

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The mechanism of the induction of choline acetyltransferase activity in the hybrid cell line NG108-15 was studied. Induction by cyclic AMP analogs, forskolin, and prostaglandin E1 + theophylline was found to be rapid with an increase in choline acetyltransferase specific activity detectable within 8 hrs and maximal after 24 hrs. Immunoblot analysis was used to demonstrate that the increase in choline acetyltransferase specific activity induced by prostaglandin E1 + theophylline was due to an increase in enzyme protein. Cycloheximide effectively blocked the induction of choline acetyltransferase by prostaglandin E1 + theophylline. These results demonstrate that the induction of choline acetyltransferase activity involves the synthesis of new enzyme protein. Attempts to measure choline acetyltransferase turnover by blocking its synthesis with cycloheximide indicated that this enzyme is a relatively stable protein with a half-life of greater than 24 hrs.

Original languageEnglish
Pages (from-to)1063-1067
Number of pages5
JournalNeurochemical Research
Volume17
Issue number11
DOIs
StatePublished - Nov 1992

Keywords

  • Choline acetyltransferase
  • NG108-15
  • cAMP
  • induction
  • neuroblastoma

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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