Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.
|Number of pages||5|
|State||Published - Aug 5 2004|
Bibliographical noteFunding Information:
This work was supported by grant nos. AG15490, AG18478, and NS039141 from NIH.
- Alzheimer's disease
- Immediate-early genes
- Primary neuronal culture
ASJC Scopus subject areas
- Neuroscience (all)