TY - JOUR
T1 - Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells
AU - Dickey, Chad A.
AU - De Mesquita, Dirson D.
AU - Morgan, Dave
AU - Pennypacker, Keith R.
N1 - Funding Information:
This work was supported by grant nos. AG15490, AG18478, and NS039141 from NIH.
PY - 2004/8/5
Y1 - 2004/8/5
N2 - Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.
AB - Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.
KW - Alzheimer's disease
KW - Immediate-early genes
KW - Induction
KW - Memory
KW - Neurotrophins
KW - Primary neuronal culture
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U2 - 10.1016/j.neulet.2004.04.089
DO - 10.1016/j.neulet.2004.04.089
M3 - Article
C2 - 15265580
AN - SCOPUS:3242668110
SN - 0304-3940
VL - 366
SP - 10
EP - 14
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -