Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells

Chad A. Dickey, Dirson D. De Mesquita, Dave Morgan, Keith R. Pennypacker

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.

Original languageEnglish
Pages (from-to)10-14
Number of pages5
JournalNeuroscience Letters
Volume366
Issue number1
DOIs
StatePublished - Aug 5 2004

Bibliographical note

Funding Information:
This work was supported by grant nos. AG15490, AG18478, and NS039141 from NIH.

Keywords

  • Alzheimer's disease
  • Immediate-early genes
  • Induction
  • Memory
  • Neurotrophins
  • Primary neuronal culture

ASJC Scopus subject areas

  • General Neuroscience

Fingerprint

Dive into the research topics of 'Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells'. Together they form a unique fingerprint.

Cite this