Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells

Chad A. Dickey; Dirson D. De Mesquita; Dave Morgan; Keith R. Pennypacker

doi:10.1016/j.neulet.2004.04.089

Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells

Chad A. Dickey
, Dirson D. De Mesquita
, Dave Morgan
, Keith R. Pennypacker

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.

Original language	English
Pages (from-to)	10-14
Number of pages	5
Journal	Neuroscience Letters
Volume	366
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2004.04.089
State	Published - Aug 5 2004

Bibliographical note

Funding Information:
This work was supported by grant nos. AG15490, AG18478, and NS039141 from NIH.

Funding

This work was supported by grant nos. AG15490, AG18478, and NS039141 from NIH.

Funders	Funder number
National Institutes of Health (NIH)
National Institute of Neurological Disorders and Stroke	R01NS039141

Keywords

Alzheimer's disease
Immediate-early genes
Induction
Memory
Neurotrophins
Primary neuronal culture

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2004.04.089

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title = "Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells",

abstract = "Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.",

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AU - Dickey, Chad A.

AU - De Mesquita, Dirson D.

AU - Morgan, Dave

AU - Pennypacker, Keith R.

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AB - Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal cultures and differentiated mouse neuroblastoma 2A (N2A) cultures reliably induces expression of several IEGs, including Zif268, Nur77 and Arc, each of which have been linked to memory consolidation. These findings provide an in vitro model in which to test other agents that might modulate the induction of memory-associated genes.

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KW - Immediate-early genes

KW - Induction

KW - Memory

KW - Neurotrophins

KW - Primary neuronal culture

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Induction of memory-associated immediate early genes by nerve growth factor in rat primary cortical neurons and differentiated mouse Neuro2A cells

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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