TY - JOUR
T1 - Induction of miR-21-PDCD4 signaling by UVB in JB6 cells involves ROS-mediated MAPK pathways
AU - Hou, Lichao
AU - Bowman, Linda
AU - Meighan, Terence G.
AU - Pratheeshkumar, Poyil
AU - Shi, Xianglin
AU - Ding, Min
PY - 2013/11
Y1 - 2013/11
N2 - Ultraviolet (UV) irradiation plays a major role in the development of human skin cancer. The present study examined the alterations of miR-21-PDCD4 signaling in a mouse epidermal cell line (JB6 P+) post exposure to UVB irradiation. The results showed that (1) UVB caused PDCD4 inhibition in JB6 cells; (2) exposure of cells to UVB caused a significant increase of miR-21, the upstream regulator of PDCD4, expression; (3) both inhibition of ERKs with U0126 and inhibition of p38 with SB203580 significantly reversed UVB-induced PDCD4 inhibition; (4) ROS scavenger, N-acetyl-l-cysteine reversed the inhibitory effect of UVB on PDCD4 expression. The above results suggested that UVB induced PDCD4 inhibition, which may be mediated through ROS, especially endogenous H2O2 and p38 and ERKs phosphorylation. Unraveling the complex mechanisms associated with these events may provide insights into the initiation and progression of UVB-induced carcinogenesis.
AB - Ultraviolet (UV) irradiation plays a major role in the development of human skin cancer. The present study examined the alterations of miR-21-PDCD4 signaling in a mouse epidermal cell line (JB6 P+) post exposure to UVB irradiation. The results showed that (1) UVB caused PDCD4 inhibition in JB6 cells; (2) exposure of cells to UVB caused a significant increase of miR-21, the upstream regulator of PDCD4, expression; (3) both inhibition of ERKs with U0126 and inhibition of p38 with SB203580 significantly reversed UVB-induced PDCD4 inhibition; (4) ROS scavenger, N-acetyl-l-cysteine reversed the inhibitory effect of UVB on PDCD4 expression. The above results suggested that UVB induced PDCD4 inhibition, which may be mediated through ROS, especially endogenous H2O2 and p38 and ERKs phosphorylation. Unraveling the complex mechanisms associated with these events may provide insights into the initiation and progression of UVB-induced carcinogenesis.
KW - JB6 cell
KW - MicroRNA-21
KW - PDCD4
KW - Reactive oxygen species
KW - Ultraviolet light
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UR - http://www.scopus.com/inward/citedby.url?scp=84885860826&partnerID=8YFLogxK
U2 - 10.1016/j.etp.2013.05.006
DO - 10.1016/j.etp.2013.05.006
M3 - Article
C2 - 23891589
AN - SCOPUS:84885860826
SN - 0940-2993
VL - 65
SP - 1145
EP - 1148
JO - Experimental and Toxicologic Pathology
JF - Experimental and Toxicologic Pathology
IS - 7-8
ER -
