TY - JOUR
T1 - Induction of peroxisomal enzymes in cultured porcine endothelial cells by the hypolipidemic drug ciprofibrate
AU - Glauert, Howard P.
AU - Hennig, Bernhard
AU - Chow, Hannah S.
PY - 1990
Y1 - 1990
N2 - The purpose of this study was to determine if the hypolipidemic peroxisome proliferator ciprofibrate, which induces peroxisomes in the liver, can induce peroxisomes in cultured porcine pulmonary endothelial cells. Ciprofibrate was added at three concentrations to cell cultures for a 6‐day period. The induction of peroxisomes in the cells was detected by determining total peroxisomal β‐oxidation and peroxisomal catalase activity. The addition of ciprofibrate was found to increase peroxisomal enzyme activities in a dose‐dependent manner, with the highest activity being reached at 1000 μM ciprofibrate. Ciprofibrate also caused an increased transfer of albumin across endothelial cells cultured on micropore filters. This study shows that peroxisomal enzyme activities can be induced by ciprofibrate in endothelial cells, which may have implications in diseases mediated by vascular injury.
AB - The purpose of this study was to determine if the hypolipidemic peroxisome proliferator ciprofibrate, which induces peroxisomes in the liver, can induce peroxisomes in cultured porcine pulmonary endothelial cells. Ciprofibrate was added at three concentrations to cell cultures for a 6‐day period. The induction of peroxisomes in the cells was detected by determining total peroxisomal β‐oxidation and peroxisomal catalase activity. The addition of ciprofibrate was found to increase peroxisomal enzyme activities in a dose‐dependent manner, with the highest activity being reached at 1000 μM ciprofibrate. Ciprofibrate also caused an increased transfer of albumin across endothelial cells cultured on micropore filters. This study shows that peroxisomal enzyme activities can be induced by ciprofibrate in endothelial cells, which may have implications in diseases mediated by vascular injury.
KW - Catalase
KW - Ciprofibrate
KW - Endothelial Cells
KW - Peroxisome
KW - β‐Oxidation
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U2 - 10.1002/jbt.2570050206
DO - 10.1002/jbt.2570050206
M3 - Article
C2 - 2283660
AN - SCOPUS:0025446044
SN - 0887-2082
VL - 5
SP - 115
EP - 118
JO - Journal of Biochemical Toxicology
JF - Journal of Biochemical Toxicology
IS - 2
ER -