Induction of peroxisomal enzymes in cultured porcine endothelial cells by the hypolipidemic drug ciprofibrate

Howard P. Glauert, Bernhard Hennig, Hannah S. Chow

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The purpose of this study was to determine if the hypolipidemic peroxisome proliferator ciprofibrate, which induces peroxisomes in the liver, can induce peroxisomes in cultured porcine pulmonary endothelial cells. Ciprofibrate was added at three concentrations to cell cultures for a 6‐day period. The induction of peroxisomes in the cells was detected by determining total peroxisomal β‐oxidation and peroxisomal catalase activity. The addition of ciprofibrate was found to increase peroxisomal enzyme activities in a dose‐dependent manner, with the highest activity being reached at 1000 μM ciprofibrate. Ciprofibrate also caused an increased transfer of albumin across endothelial cells cultured on micropore filters. This study shows that peroxisomal enzyme activities can be induced by ciprofibrate in endothelial cells, which may have implications in diseases mediated by vascular injury.

Original languageEnglish
Pages (from-to)115-118
Number of pages4
JournalJournal of Biochemical Toxicology
Volume5
Issue number2
DOIs
StatePublished - 1990

Keywords

  • Catalase
  • Ciprofibrate
  • Endothelial Cells
  • Peroxisome
  • β‐Oxidation

ASJC Scopus subject areas

  • Toxicology

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