Inflexinol inhibits colon cancer cell growth through inhibition of nuclear factor-κB activity via direct interaction with p50

Ok Ban Jung, Hoon Oh Ju, Yeon Hwang Bang, Cheul Moon Dong, Heon Sang Jeong, Seram Lee, Soyoun Kim, Hyosung Lee, Kyung Bo Kim, Bae Han Sang, Tae Hong Jin

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Kaurane diterpene compounds have been known to be cytotoxic against several cancer cells through inhibition of nuclear factor-κB (NF-κB) activity. Here, we showed that inflexinol, a novel kaurane diterpene compound, inhibited the activity of NF-κB and its target gene expression as well as cancer cell growth through induction of apoptotic cell death in vitro and in vivo. These inhibitory effects on NF-κB activity and on cancer cell growth were suppressed by the reducing agents DTT and glutathione and were abrogated in the cells transfected with mutant p50 (C62S). Sol-gel biochip and surface plasmon resonance analysis showed that inflexinol binds to the p50 subunit of NF-κB. These results suggest that inflexinol inhibits colon cancer cell growth via induction of apoptotic cell death through inactivation of NF-κB by a direct modification of cysteine residue in the p50 subunit of NF-κB.

Original languageEnglish
Pages (from-to)1613-1624
Number of pages12
JournalMolecular Cancer Therapeutics
Volume8
Issue number6
DOIs
StatePublished - Jun 2009

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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